穿心莲内酯抑制人结肠癌SW1116细胞增殖及诱导细胞凋亡作用的研究

Effect of Andrographolide in Inhibition of Human Colon Cancer SW1116 Cell

目的:观察穿心莲内酯对人结肠癌SW1116细胞生长及肿瘤细胞凋亡的影响,探讨其可能的机制。方法:用不同浓度的穿心莲内酯处理SW1116细胞,MTT检测穿心莲内酯对SW1116细胞生长增殖的抑制作用;流式细胞术(FCM)检测细胞凋亡率;比色法测定Caspase-3酶活性;Western blot法检测bax、Bcl-2的蛋白表达。结果:穿心莲内酯能够剂量依赖型的抑制人结肠癌SW1116细胞的增殖,并诱导凋亡;穿心莲内酯与SW1116细胞作用48小时后Caspase-3酶活性显著增强;同时,穿心莲内酯处理SW1116细胞后,Bax蛋白表达增强,Bcl-2蛋白表达下调。结论:穿心莲内酯可抑制人结肠癌SW1116的增殖,诱导其凋亡,机制可能与调节Caspase-3、Bcl-2和Bax表达水平有关。

Objective：To investigate the inhibitory effects of andrographolide on proliferation inhibition and apoptosis induction in human colon carcinoma SW1116 cells and its possible mechanism.Methods：The proliferation inhibition was measured by MTT assay.The flow cytometer（FCM） was used to analyze the apoptosis rate.Colorimetric method was employed to detect the activity of caspase-3.The expression of Bcl-2 and Bax proteins were measured by Western blotting assay.Results：Andrographolide inhibited SW1116 cells growth and induced their apoptosis in a dose-dependent manner.After treatment with andrographolide for 48 hours,the activity levels of caspase-3 significant increased.Furthermore,the expression levels of Bcl-2 could be decreased while the expression levels of Bax in SW1116 cells could be increased by andrographolide.Conclusions：Andrographolide can inhibit the growth and induce the apoptosis of SW1116 cells significantly,which may be related with regulating the expression levels of Caspase-3,Bcl-2 and Bax.