西安地区EV71-VP1基因分析

Genomic Analysis of Capsid Protein VP1 of Ev71 in Xi'an Area

目的:通过在原核表达系统初步构建EV71-VP1,对西安地区肠道病毒71型(EV71)的外壳蛋白VP1基因进行分析。方法:采集西安地区手足口病患儿的疱液、咽部分泌物进行病毒分离和RT-PCR检测;通过逆转录聚合酶链式反应(RT-PCR)扩增肠道病毒71型(EV71)外壳蛋白VP1基因,构建重组表达质粒PQE30/VP1,转化到大肠杆菌BL21中,对VP1基因进行遗传学分析。结果:对肠道病毒71型(EV71)西安地方株VP1基因测序,并将其与阜阳株(序列号为EU913471)相比较,表明我国西安地区EV71分离株与阜阳株有较大差别,核苷酸差异约为4%。结论:西安地方株VP1基因与阜阳株相比,其核苷酸差异较为明显。这将为西安地区EV71的分子流行病学研究以及EV71所致疾病的预防和控制,打下良好的基础。

Objective：To investigate the gene VP1 of Ev71 in Xi＇an by expressing EV71-VP1 in prokaryotic.Methods：Vesicle fluid and throat swab specimens were collected from the patients with hand-foot-and-mouth diseases,and virus was isolated.The capsid VP1 genes of EV71 were amplified by RT-PCR,the expression plasmid PQE30/VP1 was constructed and transformed to Bacillus coli BL 21.The capsid protein VP1a were analysised.Results：There was distinct difference between the EV71 strains isolated from Xi An sequenced and Fu yang（EU913471）,and difference of the nucleotide base was 4%.Conclusions：Sequence of the capsid VP1 genes of EV71 in Xi＇an area showed great difference compared with that from Fuyang,this research for Ev71 in Xi＇an provides a good basis of molecular epidemiology and the prevention and regulation.