5'-NT、血清内毒素及TNF-α在妊娠肝内胆汁淤积症患者中的表达及临床意义

Expressions of 5'-NT,serum endotoxin and TNF-α in patients with intrahepatic cholestasis of pregnancy and its clinical significance

目的:观察5’-核苷酸酶(5’-NT)、血清内毒素(LPS)及肿瘤坏死因子α(TNF-α)在妊娠肝内胆汁淤积症患者中的表达,为判断其病情严重程度及预后,寻找更有效的检测指标和治疗方法。方法:采用酶法速率法检测妊娠肝内胆汁淤积症患者(胆淤组)及健康妊娠者(对照组)血清5’-NT的活性,ELISA检测两者血清TNF-α的含量,并检测常规肝功能指标。结果:胆淤组患者血清5’-NT的活性明显高于对照组(P<0.01),胆淤组的血清TNF-α显著高于对照组,胆淤组常规肝功能指标(ALT、AST、GGT、ALP)均高于对照组,且5’-NT与ALT(r=0.763,P<0.05)、AST(r=0.684,P<0.05)均呈正相关。结论:5’-NT与妊娠肝内胆汁淤积症患者肝功能水平密切相关,TNF-α在患者体内的高表达,提示在胆汁淤积期积极抗炎治疗可能更有利于患者病情好转。

Objective：To observe the expression of 5＇-nucleotidase（5＇-NT）,serum endotoxin（LPS） and tumor necrosis factor-α（TNF-α） in patients with intrahepatic cholestasis of pregnancy,and determine their condition severity and prognosis,and find more effective detection of targets and treatment methods.Methods：The detection of enzymatic rate method was used to detect the serum 5＇-NT activity of intrahepatic cholestasis of pregnancy（ICP group） and healthy pregnancy（control group）,and both serum endotoxin and TNF-α levels were measured by ELISA,and to test liver function.Results：The serum 5＇-NT activity and TNF-α level of ICP group was significantly higher than control group,and liver function of ICP group（ALT,AST,GGT,ALP） were higher than control group,and the 5＇-NT and ALT（r = 0.763,P〈0.05）,AST（r = 0.684,P〈0.05） were positive correlation.Conclusion：5＇-NT levels of intrahepatic cholestasis of pregnancy in patients is closely related to their liver function,the level of serum TNF-α in patients was high in vivo,its suggested that the period of active anti-inflammatory treatment of ICP may be more conducive to patient＇s condition improved.