Neuritin诱导大鼠骨髓基质细胞分化为神经元样细胞的电生理研究

Electrophysiology Study on Differentiation of Rat Bone Marrow Stromal Stem Cells into Neuron-like Cells by Neuritin

目的:探讨神经营养因子Neuritin诱导大鼠骨髓基质细胞分化为神经元样细胞的电生理特性。方法:应用膜片钳技术,采用全细胞记录方式,对由Neuritin诱导的大鼠骨髓基质细胞进行诱导前后的电生理功能测定。结果:分化后的神经元样细胞较诱导前细胞的膜特性[静息膜电位(RMP)膜电容(Cm)串联电阻值(Rs)]有了显著改变(p<0.01)。分化后细胞记录到K+电流,包括两种成分:外向延迟整流K+电流和内向整流K+电流。结论:骨髓基质细胞经过Neuritin诱导能够向功能性神经元方向分化。

Objective：To explore the electrophysiological properties of differentiation of rat bone marrow stroma cell to Neuritin by neurotrophic factor.Methods：electrophysiological functions of rat bone marrow stroma cell before and after differentiating into neurons induced by Neuritin.The membrane currents and resting membrane potentials were recorded by using whole cell patch clamp technique.Results：The membrane properties （RMP,Cm,Rs） changed a lot between differentiated cell and undifferentiated neuron-like cell.（P0.01）.The cell recorded K＋ current flow after differentiate and contained two components：the delayed outwardly rectifying K＋currents and the inwardly rectifying K＋ currents.Conclusion：Marrow stroma cell （MSC） can differentiate into functional neurons if induced by Neuritin.