不同光、温条件下野生宿主对噬藻体PP的光修复率

The photoreactivation ratio of cyanophage PP in wild host under different light or temperature conditions

研究了1株野生宿主藻对UV-B损伤的噬藻体PP的光修复率,结果显示该野生宿主的光修复率显著高于实验室培养的坑形席藻(Phormidium foveolarumIU427)和鲍氏织线藻(Plectonema boryanum IU594)。不同理化条件(光质、光强、水温)下该野生宿主对经UV-B损伤的噬藻体PP光修复情况,结果表明:野生宿主的光修复率与UV-A强度、可见光强度、水温之间均呈显著正相关(P<0.05);可见光所驱动的光修复能力明显高于UV-A所驱动的光修复,且修复率会在可见光强达到160μE.m-.2s-1时接近饱和。说明,自然条件下浅水湖泊中可见光介导的宿主光修复作用占主导地位,由于受水温和透明度的双重影响,野生宿主在秋季的修复能力最强,而在冬季的修复能力最弱。

The photoreactivation ratio of cyanophage PP in its wild host was studied.The results indicate that the photoreactivation ratio in wild host was significantly higher than those of cultured hosts（Phormidium foveolarum IU427 and Plectonema boryanum IU594）.Besides,the influences of ecological factors,including light quality,light intensity and water temperature,on the photoreaction ratio in wild host were also studied.The results indicate that the photoreactivation ratio was positively correlated with UV-A intensity,visible light intensity and water temperature（P〈0.05）.Moreover,the photoreactivation ratio caused by visible light was significantly higher than that caused by UV-A,and the photoreactivation ratio could approach its maximum when the visible light intensity reached 160μE·m^-2·s^-1.Our results suggest that visible light may be the dominant power for photoreactivation in shallow lakes,and the photoreactivation ability of wild host may be weakest in winter and strongest in autumn as far as the combined effect of water temperature and transparency was concerned.