优雅蝈螽与暗褐蝈螽精子束的显微观察

Microscopic observation of spermatodesms of Gampsocleis gratiosa and G. sedakovii ( Orthoptera: Tettigoniidae)

本文应用微分干涉相衬法对优雅蝈螽Gampsocleis gratiosa Brunner von Wattenwyl和暗褐蝈螽G.sedakovii(Fischer von Waldheim)雄性精巢管基部、输精管、贮精囊和精包,及雌性受精囊中精子束的形态变化进行了观察,对探讨螽斯近缘种的生殖隔离机制和生殖生物学具有重要意义。结果表明:这两种蝈螽的精子束通过精包转移到雌性受精囊后,精子束的形态发生了显著变化。精巢管基部的精子为游离的单个精子;输精管、贮精囊和精包中精子成束排列形成较分散的精子束,精子束头部包裹有粘液帽;雌性受精囊中的精子束的精子呈羽状排列,精子的头部汇集在中央轴上。两种蝈螽精子束形态差异不显著。

Spermatodesms of Gampsocleis gratiosa and G. sedakovii was studied by differential interference contrast （ DIC） microscope,which is of significance to study the reproductive isolation mechanism and reproductive biology of the related species. The results indicate that the spermatodesms of G. gratiosa and G. sedakovii,which are transferred from the male to the female by spermatophores,are considerably different between the male and the female even in the same species. In testis the sperm is single. In vasa deferentia,seminal vesicles and spermatophores, sperms are grouped together in bundles to form spermatodesms. There is a mucous cap that encloses the head of the spermatodesms. In female spermatheca,the spermatodesms are feather-shaped with a median axis. The morphology of spermatodesms shows little difference between the two species.