终末期肾病患者肿瘤坏死因子α基因多态性与微炎症状态的相关性研究

Relationship between tumor necrosis factor alpha gene polymorphism and microinflammatory state in end-stage renal diseases

目的探讨肿瘤坏死因子(TNF-α)基因G-308A位点的遗传多态性和终末期肾病(ESRD)患者微炎症状态的相关关系。方法采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)检测30例健康对照者、60例终末期肾病患者该位点的基因多态性;应用ELISA测定血清C反应蛋白(CRP)、TNF-α浓度。结果 TNF-α-308AG+AA基因型在ESRD组、健康对照组分布频率分别为12%、7%,两组在基因型分布频率、等位基因频率差异均无统计学意义。ESRD组的不同基因型血清CRP、TNF-α浓度未发现差异;ESRD患者血清CRP、TNF-α浓度与基因型之间的Spearman相关系数分别为0.101和0.09(P>0.05)。结论 TNF-α基因G-308A位点的基因多态性与ESRD的发生无相关性。ESRD患者血清CRP、TNF-α浓度升高与TNF-α基因G-308A位点的基因多态性无关。

Objective To explore the relationship of between tumor necrosis alpha （TNF-α） gene G308A polymorphism and microinflammatory state in end-stage renal diseases （ESRD）.Methods The G-308A polymorphism of TNF-α promoter was genotyped by using PCR-RFLP method in 60 patients with ESRD and 30 cases of control of Han ethnic group in Guangdong ; the serum levels of C-reactive protein （CRP）, TNF-α were determined using ELISA assay.Results The frequency of AG＋AA genotypes of TNF-α-308 appears to be 12% in the ESRD group, and 7% in the control.No significant difference was found in genotype distribution and allelic frequency between the ESRD group and the control.The serum levels of CRP and TNF-α in the ESRD group showed no difference between GG genotype and AG＋AA genotype.It showed that there was no significant correlation between the genotypes and the serum levels of CRP （r=0.101） or TNF-α （r=0.09） using the Spearman＇s rank correlation.Conclusion TNF-α gene G308A polymorphism is not associated with ESRD and don＇t contribute to the increased serum levels of CRP and TNF-α in the ESRD patients.