松材线虫伴生细菌多样性的宏基因组分析

Diversity of bacteria associated with pine wood nematode revealed by metagenome

【目的】松材线虫是松材线虫病的病原,且与其伴生细菌之间存在互作关系,它们构成一个微生态系统。本研究旨在揭示松材线虫-伴生细菌群落细菌多样性。【方法】采用16S rRNA基因文库和454测序对伴生细菌群落的宏基因组进行初步分析。【结果】依据97%序列相似性划分OTU(Operational Taxonomic Unit),构建的16S rRNA文库包含25个OTU,分别属于Alphaproteobacteria、Betaproteobacteria、Gammaproteobacteria和Bacteroidetes,其中优势菌群为Gammaproteobacteria,特别是Stenotrophomonas maltophilia为优势细菌。在伴生细菌优势种群上,454测序结果与16SrRNA基因文库结果基本一致。【结论】松材线虫的伴生细菌多样性较高,这些细菌可能对松材线虫具有一定的生态意义。

[Objective ] The pine wood nematode （PWN）,Bursaphlenchus xylophilus,which collaborates with its associated bacteria to form ecosystem and has interaction among them,is the pathogen of pine wilt disease.This study focused on revealing the bacterial diversity of ecosystem of pine wood nematode and its associated bacteria.[Methods] The metagenome of ecosystem of bacteria associated with the PWN was analyzed by 16S rRNA gene library and 454 sequencing.[Results]The results showed that 25 OTUs（Operational Taxonomic Units） were obtained from the library according to sequences similarity of 97%,which affiliated to Alphaproteobacteria,Betaproteobacteria,Gammaproteobacteria and Bacteroidetes.The dominant bacteria were belonged to Gammaproteobacteria,especially Stenotrophomonas maltophilia in this class dominated the library.In terms of dominant bacteria,the results revealed by metagenome were similar to that of 16S rRNA gene library.[Conclusion]The diversity of bacteria associated with the PWN is high and these bacteria maybe have ecological role to the PWN.