不同培养条件对胶质芽孢杆菌诱导碳酸钙晶体形成的影响

Effect of different culture conditions on carbonic anhydrase from Bacillus mucilaginosus inducing calcium carbonate crystal formation

【目的】研究不同培养条件对胶质芽孢杆菌(Bacillus mucilaginosus)菌体形态、数量和分泌的碳酸酐酶(CA酶)活性的影响,以及不同方式培养的菌体与碳酸钙晶体的生长及其形貌、数量之间的联系。【方法】分别采用无氮和有氮培养基培养胶质芽孢杆菌,进行菌体形态、数量及CA酶活性的比较,收集不同培养方式的菌体加入碳酸钙结晶体系中以研究细菌与碳酸钙晶体形成的联系。【结果】在无氮培养条件下,胶质芽孢杆菌数量少、荚膜肥厚,细菌培养液CA酶活力较低;有氮培养条件下,菌体数量多、荚膜单薄,细菌培养液CA酶活力较高。在碳酸钙结晶体系中加入无氮培养的菌体,生成的碳酸钙晶体表面光滑,体积较大但数量较小,加入有氮条件下培养的菌体形成的碳酸钙晶体表面粗糙,数量大但体积较小。【结论】不同培养条件能够引起胶质芽孢杆菌菌体数量、荚膜多糖及CA酶活的明显差异,进而对碳酸钙晶体的生成和形貌产生影响。

[Objective]Effect of various culture conditions on the morphology,amount and carbonic anhydrase （CA） activity of Bacillus mucilaginosus were examined,as well as the effect on calcium carbonate crystal forming,shape and amount.[Methods]The strain was inoculated in N-free or N-containing medium,and the bacterial morphology,number and CA activity were compared under different culture conditions.By collecting different cultures and adding them to the system of calcium carbonate crystallization we studied the relationship between the bacteria and the formation of calcium carbonate crystals.[Results]A small number of cell,capsular hypertrophy,lower CA activity in bacterial culture were obtained under N-free culture condition.In contrast,more biomass quantity,thin capsule,and high CA activity were got in the nitrogen-containing culture.In the calcium carbonate crystal system,adding N-free culture of bacteria produced a smooth surface of calcium carbonate crystals,larger volume but small density,the addition N-containing culture of bacteria formed rough surface,bigger density but smaller volume of calcium carbonate crystals.[Conclusion]Different culture conditions can cause significant differences in bacterial amounts,capsular thickness and CA activity,and then influence the crystal growth and form of calcium carbonate.