甜瓜抗白粉病基因SRAP分子标记筛选

A Novel SRAP Marker of the Resistant Gene for Powdery Mildew in Melon

以感白粉病甜瓜A120和抗白粉病甜瓜A119为亲本构建F2代分离群体,采用BSA和SRAP相结合的方法筛选与甜瓜抗白粉病基因相连锁的分子标记。结果显示,294条SRAP引物中有6条引物在抗病与感病池间表现出多态性;对8个高抗和8个高感单株进行扩增,引物me46em51和me3em6分别在高感单株中扩增出210 bp和205 bp的多态性条带,而高抗单株无此扩增带,与抗病、感病池结果一致。采用JoinMap3.0软件进行连锁分析,两标记与抗白粉病基因的连锁距离分别为18.2 c M和23.4 c M,初步推测本实验中甜瓜白粉病抗性为隐性多基因控制。

The mapping population was constructed with the susceptible melon A120 and the resisant melon A119 as parents.The markers of resistance to powdery mildew were screened with the BSA and SRAP technique.The results showed that 6 primers of 294 SRAP primers could detect the polymorphism between the resistant pool and susceptible pool.No band were detected in 8 high-resistant individuals and signature band of 210 bp and 205 bp were amplified respectively in 8 high-susceptible individuals with me46em51 and me3em6,which was correspondent to the results in resistant and susceptible pool.Based on the linkage analysis by JoinMap3.0,the distance between the two markers with the powdery mildew resistance gene were 18.2 cM and 23.4 cM separately.It is preliminarily deduced that powdery mildew resistance in melon is controlled by polygene recessive genes.