3种生态型翅果油树种子贮藏蛋白分析

Analysis of Seed Storage Protein in Three Ecotypes Elaeagnus mollis Diels

采用考马斯亮蓝G-250染色法和SDS-PAGE电泳技术对翅果油树3种生态类型种子贮藏蛋白含量进行测定和图谱分析。结果显示:大宫灯、长果型、小宫灯3种生态类型种子中总贮藏蛋白含量分别为33.753%、32.075%和26.633%;3种生态类型种子贮藏蛋白均以谷蛋白含量最高(65.971%～68.267%),其次为球蛋白和清蛋白,醇溶蛋白的含量最低;电泳图谱显示,3种生态类型之间清蛋白、球蛋白、醇溶蛋白和谷蛋白的蛋白质条带信息均存在差异。研究表明,翅果油树3种生态类型间种子贮藏蛋白具有多态性。

Seed storage proteins of three ecotypes in Elaeagnus mollis Diels were studied on the content determination and electrophoresis analysis by using the staining with Coomassie brilliant blue G-250 and a sodium dodecylsulfate electrophoresis performed polyacrylamide gels（SDS-PAGE）.The results indicated that the percentage content of total storage proteins in the seed of three ecotypes,which are named Dagongdeng,Changguoxing and Xiaogongdeng,is respectively 33.753%,32.075%,26.633%.For Elaeagnus mollis diels,most of storage proteins are glutelin,and the next is albumin and globulin,respectively.There is a little alcohol soluble protein.There are significant differences in the contents of total storage proteins between three ecotypers.Electrophoresis maps reveral that three ecotypers are differentiated by the information of protein subunits on glutelin,albumin,globulin and alcohol soluble protein.In other words,seed storage proteins display obvious polymorphisms among three ecotypes.