摘要
目的建立复方抗疲劳缓释片中人参皂苷Re的含量测定方法。方法采用高效液相色谱法,固定相:Shim-pack CLC-ODS(4.6mm×250mm,5μm),流动相:乙腈-0.5%磷酸溶液(21∶79),柱温:26℃,流速:1.0ml/min,紫外检测波长:203nm。结果人参皂苷Re在0.01998~0.3996mg/ml范围内线性关系良好,标准曲线方程:A=7.173×106C-26219,r=0.9999(n=6),平均加样回收率为104.2%(n=9),RSD为1.03%。结论该方法简便、快捷、准确,可用于复方抗疲劳缓释片中人参皂苷Re的含量测定。
Objective To establish a high performance liquid chromatography method for determination of ginsenoside Re in Compound Antifatigue Sustained-release Tablets.Methods A high performance liquid chromatography method was developed.A kromasil C 18 column was used with a mobile phase of acetonitrile-0.5%phosphoric acid(21∶ 79).The UV absorbance was monitored at 203 nm.Results The linear range of ginsenoside Re was 0.019 98-0.3996 mg/ml,A=7.173×106C-26 219(r=0.9999,n=6),The mean recovery was 104.2%(n=9),RSD=1.03%.Conclusion The method is proved to be simple,fast and accurate,and can be used for determination of ginsenoside Re in Compound Antifatigue Sustained-release Tablets.
出处
《解放军药学学报》
CAS
2010年第3期250-251,254,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
高效液相色谱法
复方抗疲劳缓释片
人参皂苷RE
含量测定
high performance liguid chromatography
Compound Antifatigue Sustained-release Tablet
ginsenoside Re
content determination