复方抗疲劳缓释片中人参皂苷Re的含量测定

Determination of Ginsenoside Re in Compound Antifatigue Sustained-release Tablets

目的建立复方抗疲劳缓释片中人参皂苷Re的含量测定方法。方法采用高效液相色谱法,固定相：Shim-pack CLC-ODS（4.6mm×250mm,5μm）,流动相：乙腈-0.5%磷酸溶液（21∶79）,柱温：26℃,流速：1.0ml/min,紫外检测波长：203nm。结果人参皂苷Re在0.01998~0.3996mg/ml范围内线性关系良好,标准曲线方程：A=7.173×106C-26219,r=0.9999（n=6）,平均加样回收率为104.2%（n=9）,RSD为1.03%。结论该方法简便、快捷、准确,可用于复方抗疲劳缓释片中人参皂苷Re的含量测定。

Objective To establish a high performance liquid chromatography method for determination of ginsenoside Re in Compound Antifatigue Sustained-release Tablets.Methods A high performance liquid chromatography method was developed.A kromasil C 18 column was used with a mobile phase of acetonitrile-0.5%phosphoric acid（21∶ 79）.The UV absorbance was monitored at 203 nm.Results The linear range of ginsenoside Re was 0.019 98-0.3996 mg/ml,A=7.173×106C-26 219（r=0.9999,n=6）,The mean recovery was 104.2%（n=9）,RSD=1.03%.Conclusion The method is proved to be simple,fast and accurate,and can be used for determination of ginsenoside Re in Compound Antifatigue Sustained-release Tablets.