战骨总黄酮提取工艺及不同部位含量测定研究

Extraction Condition and Quantitative Analysis of Flavonoid in Different Sections of Premna fulva Craib

采用单因素试验和正交试验相结合的方法确定了超声波辅助提取战骨总黄酮的最佳工艺条件，即：超声波功率120W、频率35kHz、乙醇浓度40％、固液比1：16、提取时间90min。测定了战骨不同器官、不同部位及不同粗度茎中总黄酮含量，结果表明，总黄酮含量最高的器官是叶，最低的是根，茎中含量居中；直径为0．5～0．9cm及1．6～1．8cm的茎中总黄酮含量较高；在根与茎中，黄酮类化合物主要分布于皮部。该提取方法稳定、重现性好，精密度和回收率高。

By studying the extraction technology and the content by orthoronal design,the optimum extracting conditions for univariate of the flavonoid in Prernna fulva craib were determined： ultrasonic power 120 W, ultrasonic frequency 35 kHz, the proportion of solid and liquid 1/16,extracting time 90 min, extraction reagent 40% alcohol. A conclusion can be drawn that there were more contents of flavonoids in the leaves than in stems and roots and epidermis contained more flavonoid. The stability and reappear probability of the method were high ,so were the precision and the recovery rate.