摘要
目的:研究黄芩苷对兔tenon囊成纤维细胞增殖及TGF-β1表达的影响,初步探讨其对青光眼术后滤过瘢痕的治疗作用。方法:以体外培养的兔tenon囊成纤维细胞为实验对象,分别加入含不同浓度(15,30,45,60μg/m1)黄芩苷培养24,48,72h后采用MTT法检测药物对细胞的抑制率;采用流式细胞术及半定量逆转录聚合酶链反应法(RT-PCR)检测30μg/ml黄芩苷作用48h后成纤维细胞周期情况及TGF-β1的mRNA的变化;结果:MTT法显示黄芩苷各实验组与对照组抑制率间比较差异有显著性;流式细胞术显示黄芩苷将细胞阻抑于G1期;RT-PCR显示药物作用后细胞中TGF-β1的mRNA含量显著降低。结论:黄芩苷可抑制体外培养的兔Tenon囊成纤维细胞增殖,其作用可能跟下调TGF-β1的表达有关。
Objective：To explore the inhibitory effects of the Baicalin on the proliferation of cultured rabbit tenon＇s capsule fibroblasts（HTFs）.Methods：MTT assay was used to examine the effect of baicalin on the proliferation of the fibroblasts in different time,while the cell cycle was analyzed by FCM.TGF-β1,mRNA was half-quantitatively analyzed by a modified RT-PCR.Results：Proliferation of fibroblasts were suppressed by baicalin from a dose of 15μg/ml to 60μg/ml（P〈0.05）.FCM revealed that fibroblasts population was significantly increased at the GO/Glphase and decreased at the S phase after the baicalin treatment.RT-PCR showed that the transcription of TGF-β1 mRNA was up-regulated significantly in the experimental group compared with the control group.Conclusion：The findings suggested that baicalin can inhibit fibroblast proliferation and may be beneficial to patients with ocular fibroliferative disorders.
出处
《激光杂志》
CAS
CSCD
北大核心
2010年第3期86-87,89,共3页
Laser Journal
