用离体培养无性繁殖苦瓜

IN VITRO CLONAL PROPAGATION OF BALSAM PEAR ( MOMORDICA CHARANTIA L.)

切取由苦瓜种子形成的试管苗的顶芽及带芽茎段，移植于附加４～６ｍｇ／ＬＺＴ或４～６ｍｇ／Ｌ６－ＢＡ的ＭＳ培养基上，可以促进芽的增殖，形成芽簇，每月每株可增殖４苗；移植于附加４ｍｇ／ＬＺＴ及０．５ｍｇ／ＬＫＴ或附加４ｍｇ／ＬＺＴ及ＬＨ（水解乳蛋白）的培养基上，每月每株可增殖５苗．将幼苗接转于ＭＳ＋ＺＴ０．５ｍｇ／Ｌ的培养基上，生长健壮．丛芽生根选用大量元素减半的ＭＳ培养基，蔗糖浓度为２％效果最好，生根率达８０％，移栽成活率为８０％．

The bud explant,cut off from Balsam pear stelile seedlings,were cultured on MS media supplemented with 4～6mg/L of 6 BA or 4～6mg/L of ZT,which stimulated proliferation of the bud meristems to form bud clusters,the propagation co efficient reached 4;the bud explants were cultured on MS medium 4mg/L ZT+ 0.5mg/L KT or 4mg/L ZT+50mg/L LH,the propagation co efficient reached 5 or above.The buds were transferred to medium MS+ZT 0.05mg/L,and rootless plantlets were obtained.On medium MS containing 1/2 macronutrient elements,sucrose 2%,the plantlets began to root in 10 days,the frequency of root organogenesis reached 80%.The transplanting survival rate was 80%.