少棘蜈蚣毒素多肽SsmTx1全长cDNA的克隆和序列分析

Cloning and Characterization of a Novel Full-Length cDNA Sequence of Venom Peptide SsmTx1 from the Centipede Scolopendra subspinipes Mutilans

构建了少棘蜈蚣毒腺组织cDNA文库.运用PCR方法从少棘蜈蚣(Scolopendra subspinipes mutilans)毒腺组织cDNA文库中筛选到一个全新的毒素多肽cDNA序列,命名为SsmTx1.SsmTx1全长为417 nt,3′和5′非编码区(UTR)分别为120 nt和54 nt,其加尾信号aataaa在距离poly(A)上游16 nt.SsmTx1序列含有一个240nt的开放阅读框(ORF),共编码80个氨基酸残基,其中包括25个氨基酸的信号肽序列和55个氨基酸的成熟毒素多肽序列,含有2对二硫键.SsmTx1毒素基因是从少棘蜈蚣毒中分离鉴定的全长毒素多肽.

A cDNA library was successfully constructed from the venom gland of the centipede Scolopendra subspinipes mutilans.A novel centipede toxin gene,named SsmTx1,was isolated and characterized from the venom gland cDNA library of the centipede Scolopendra subspinipes mutilans by PCR method.The full-length cDNA sequence of SsmTx1 has 417 nt length,including 120 nt 3′ non-coding region（UTR） and 54 nt 5′ non-coding region.Its signal tail aataaa lies at the 16 nt upstream of poly（A） tail.The cDNA sequence contains a 240 nt open reading frame（ORF） with a total encode of 80 amino acid residues including 25 amino acid residues of the signal peptide and 55 amino acid residues of the mature peptide bridged by two pairs of disulfide bonds.SsmTx1 is the first full-length toxin gene from the centipede Scolopendra subspinipes mutilans.