褪黑素对急性肾脏缺血再灌注损伤模型小鼠血红素加氧酶-1表达的影响

Effects of Melatonin on HO-1 Expression in Mice with Acute Renal Ischemia-reperfusion Injury

目的:研究褪黑素(MT)对急性肾脏缺血再灌注损伤模型小鼠的保护作用及其机制。方法:将昆明种小鼠40只,随机分成MT高、低剂量(10、1 mg.kg^(-1))组,假手术组,缺血再灌注组,造模前30 min各组分别腹腔注射相应药物,后2组注射等容量的3%乙醇生理盐水。造模24 h后测定小鼠血清肌苷(Cr)与尿素氮(Bun)水平,观察肾脏组织的病理学改变,免疫组织化学方法观察小鼠肾脏血红素加氧酶-1(HO-1)的表达。结果:与假手术组相比,缺血再灌注组肾小管上皮细胞呈明显的缺血性改变,血清Cr与Bun水平均显著性升高(P<0.01),肾脏HO-1表达明显增强;与缺血再灌注组相比,MT高、低剂量组肾小管上皮细胞缺血性改变减轻,血清Cr与Bun水平均显著性降低(P<0.01),肾脏HO-1表达明显增强。结论:MT可能通过上调HO- 1的表达而促进其合成从而发挥对急性肾脏缺血再灌注损伤模型小鼠的保护作用。

OBJECTIVE：To study protection effect of melatonin（MT） on mice with acute renal ischemia-reperfusion injury（IRI） and its mechanism.METHODS：A total of 40 Kunming mice were divided into MT high-dose and low-dose groups（10,1 mg·kg^-1）,sham operation group,IRI group.Four groups were all injected with relevant drug intraperitoneally 30 min before modeling.Last two groups received 3% ethanol normal saline.The level of serum cretinine（Cr） and Bun were determined,and renal pathologic changes were observed 24 hours after modeling.The heme oxyenase-1（HO-1） expression of kidney was evaluated by immunohistochemistry.RESULTS：Compared with sham operation group,some ischemic changes of renal tubular epithelical cells was observed,and the content of Cr and Bun（P〈0.01） and the HO-1 expression increased significantly in IRI group.Compared with IRI group,some mild ischemic changes of renal tubular epithelical cells was observed,the contents of Cr and Bun were decreased（P〈0.01） while the HO-1 expression was increased significantly in MT high-dose and low-dose groups.CONCLUSION：MT could increase HO-1 expression to protect mice with IRI.