赤灵芝 GL-2 菌株深层培养复因子筛选

Selection of Multi Factor in Submerged Culture of GL 2

为筛选赤灵芝ＧＬ－２菌株深层培养的高产组合，以多糖产量为目标，进行了五因素四水平及四因素三水平的正交设计试验．结果表明，赤灵芝ＧＬ－２深层培养中，Ｃ源种类和含量是影响灵芝多糖产量的主要因素，其适用Ｃ源为麦芽糖与葡萄糖（３．５％∶０．５％），适用Ｎ源为蛋白胨与酵母膏（０．５％∶０．５％）．

The orthogonal designed experiments of five factors, four levels and four factors, three levels were carried out in order to select a high quantity combination for Ganoderma Polysaccharide by submerged culture. The results show that carbon source has a great influence on the quantity of Ganoderma Polysaccharide and the optimum carbon source, nitrogen source are maltase and glucose(3.5%∶0.5%), peptone and yeast extraction(0.5%∶0.5%) respectively.