沙利度胺抑制高脂诱导的炎性因子表达

Thalidomide Inhibits the Expression of Inflammation Factors Induced by High Lipid Diet

目的研究沙利度胺对高脂诱导的炎症因子表达的影响,进一步明确其抗动脉粥样硬化(As)的机制。方法健康新西兰兔36只,随机分为正常组、高脂组、沙利度胺组,酶联免疫吸附法(ELISA)检测血液中肿瘤坏死因子α(TNF-α)、白细胞介素8(IL-8)和IL-1水平;罗氏生物化学仪检测总胆固醇(TC)、高密度脂蛋白(HDL)、甘油三酯(TG)含量。苏丹IV染色检测主动脉As病变面积,HE染色检测As病变程度。结果高脂组TNF-α、IL-8和IL-1β水平比正常组显著升高(P<0.05)。沙利度胺组TNF-α、IL-8和IL-1水平比高脂组显著降低(P<0.05)。高脂组和沙利度胺组TC、HDL和TG较正常组明显升高,但两者之间差异无显著性。苏丹IV染色和HE染色发现高脂组有明显的As病变存在,沙利度胺组As病变面积和病变程度都明显减轻。结论沙利度胺通过抑制高脂诱导的炎性因子表达而非降脂效应抑制As的形成。

Objective To study the influence of thalidomide （ Thd ） on the expression of inflammatory factors, and explore the mechanism of thalidomide inhibiting atherosclerogenesis. Methods After serum collected, TNF - α,IL - 8 and IL - 1 levels were analyzed by ELISA kits, and total cholesterol （ TC ）, high density lipoprotein （ HDL）, and triglyceride （ TG ） were detected by kits too. After the whole aorta was free, Sudan IV staining was used to visualize the atherosclerotic plaque area. HE staining was used to define the character of atherosclerotic plaque. Results Inflammatory response was induced by high lipid diet, which was confirmed by high level TNF - α, IL - 8 and IL - 1 in high lipid diet group compared with normal diet group. The expression of inflammatory factors was inhibited by thalidomide obviously, which was confirmed by low level TNF - α, IL - 8 and IL - 1 in high lipid diet group compared with high lipid diet added with drug group. Compared with high cholesterol group, the content of TC, LDL, HDL, TG in Thd group had no statistical difference. Athesosclerotic lesions existed in high lipid diet group. The size and the degree of athesosclerotic lesions decreased in high lipid diet added with drug group compared with high lipid diet group. Conclusion Thalidomide can inhibit the expression of inflammatory factors, which may be the important mechanism of thalidomide inhibiting atherosclerogenesis.