自体荧光和肿瘤的光谱诊断研究进展

Research Progress on Autofluorescence and Spectral Diagnosis of Tumors

组织的自体荧光可用于肿瘤的光谱诊断.用不同波长的激光激发组织获得不同的自体荧光光谱特征.它们分别起源于蛋白质(色氨酸)、卟啉-蛋白的能量转移、以及内源卟啉的荧光等.通过促进卟啉的生物合成,可以增强自体荧光;采用时间门光谱技术,可以提高自体荧光诊断的灵敏度.光诱导的荧光变化的探测可以提供光动力产生的单态氧和以卟啉为基础的光动力治疗效率的信息.

Autofluorescence of tissues can be used for spectral diagnosis of tumors. Different spectral features are obtained when they are excited by laser light at different wavelengths. The origin of their spectrum are protein (tryptophan). energy transfer between protein and fluorophore protoporphyrin. and endogenous protoporphyrin respectively. Autofluorescence of tissues can be enhanced by stimulating biosynthesize of endogenous, th detection sensitivity of autofluo-rescence diagnosis can be enhanced by using time-gate spectroscopy techniqul. Detection of photoinduced fluorescence changes can provide information on photodynamically generated singlet oxygen and on the efficiency of prophyrin-based photodyuamic therapy (PDT).