2007年上海市部分嗜肺军团菌血清1型菌株PFGE分型研究

Genotyping Study on Some Strains of Legionella Pneumophila Serotype 1 in Shanghai by Pulsed-field Gel Electrophoresis

[目的]研究2007年上海市公共场所部分空调冷却塔水中分离出来的嗜肺军团菌1型(Lp1)的基因特征。[方法]从空调冷却塔水中检测到26株Lp1嗜肺军团菌后,使用脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)技术对酶切后的Lp1军团菌全染色体DNA进行电泳获得指纹图谱,并用BioNumerics软件对其进行聚类分析。[结果]26株Lp1军团菌可分为15个PFGE型。菌株间相似性系数在30.00%～100.00%之间不等,大部分菌株的PFGE型别差异明显,无优势型别。大部分PFGE型别的菌株来源场所单一;而大部分同一场所来源的军团菌菌株为相同的PFGE型。[结论]2007年上海市公共场所部分空调冷却塔水中分离出来的Lp1军团菌呈现基因多样性。

[ Objective ] To study the genotypic characteristics of some Legionella pneumophila serotype 1 （ Lpl ） isolated from cooling tower water in public settings of Shanghai in 2007. [ Methods] Twenty-six environmental isolates, which were isolated from the cooling tower water, were identified as Lpl. The pulsed-field gel electrophoresis（ PFGE ）was applied to draw the fingerprintings of the isolates, while the cluster analysis was employed hy BioNumerics software. [ Results ] Fifteen distinguishable PFGE patterns were differentiated among 26 Lp1 strains. Most of the strains differed in genetic similarity, from 30.00% to 100.00%, with no predominant PFGE pattern. The majority of PFGE patterns were composed of the isolates from the same place, while most of the strains from same source were indentified as same PFGE pattern. [ Conclusion ] Great genotypie diversity was found in Lpl isolated from cooling tower water in public settings of Shanghai in 2007.