人特异性蛋白激酶-15基因在药物性牙龈增生及正常牙龈组织中表达的研究

Expression of NYD-SP15 gene both in DGO and normal gingival tissue

目的检测细胞增殖相关基因人特异性蛋白激酶-15(specific protein kinase 15,NYD-SP15)基因在药物性牙龈增生(drug-induced gingival over-growth,DGO)及正常牙龈组织中的表达。方法应用RT-PCR法检测13例药物性牙龈增生患者牙龈切除术中切除的增生牙龈组织标本(A组:钙通道阻滞剂,B组:抗癫痫药物,C组:免疫抑制剂)及20例正常牙龈组织(取自外伤冠折后行牙冠延长术中切除的牙龈组织)中NYD-SP15基因的表达。结果药物性牙龈增生组织中NYD-SP15的mRNA水平高于正常牙龈组织(P<0.05)。在增生牙龈组织中,各药物组间NYD-SP15基因的表达量无统计学差异(P>0.05)。结论 NYD-SP15的异常表达在人药物性牙龈增生发生发展过程中起到一定的作用,但该基因高表达与药物性牙龈增生发生的相关机制尚待进一步研究。

Objective To investigate the relevance of human specific protein kinase 15 （NYD-SP15）gene and drug-induced gingival over-growth（DGO） by detecting the expression differences of NYD-SP15 gene in gingival tissue both of DGO patients and normal people. Methods Reverse transcription-polymerase chain reaction（RT-PCR） was applied to detect the expression of NYD-SP15 gene in gingival tissue both of 13 cases of DGO patients and 20 cases of normal people. The DGO patients were divided into three groups（ A： group of calcium channel blockers, B ： group of anti-epileptic drugs, C ： group of immunosuppressants）, and the samples were the exci- sion of gingival tissue during gingivectomy. The normal gingival tissue was the excision of gingival tissue during crown lengthening sur- gery （ the teeth were traumatic crown fractures）. Results The expression of NYD-SP15 in tissue of DGO was significantly higher than that of normal gingiva tissue （P 〈 0.05）. And there was no significant difference between the three DGO groups（ P 〉0.05） . Conclusions Abnormal expression of NYD-SP15 plays an important role in DGO. However,the relationship between the gene and the mechanisms of DGO remains to be further studied.