实时荧光PCR检测在乙型肝炎病毒DNA检测方面的临床意义

Clinical Significance of Hepatitis B Virus DNA Detected by Real-time Fluorescence PCR

目的对采用血清学标志物检测确定为乙型肝炎的患者进行实时荧光PCR检测,探讨实时荧光PCR检测的临床应用价值。方法对乐昌市人民医院自2007年7月到2009年12月门诊及体检中心进行血清学标志物检测(HBV-M)诊断为阳性的乙型肝炎患者336例,采取实时荧光PCR检测,对其结果进行分析。结果大部分乙型肝炎患者定量PCR检测HBV-DNA的结果与HBV血清学标志物检测结果一致。大三阳、小三阳患者血清中PCR结果拷贝数均较高,而HBsAb(+)或HBsAg(-)的标本PCR有个别阳性结果 ,但其拷贝数很低。结论实时荧光PCR检测HBV-DNA在判断体内HBV是否在复制,复制程度以及HBV血清学标志阴性肝炎患者的诊断方面,较优于血清学标志物检测,值得临床推广。

Objective Ascertains the value carrying out real time fluorescence PCR detecting, discussing the clinical practice that real time fluorescence PCR checks for the hepatitis B patient to adopt serum to learn to indicate thing detecting. Method Directs on Lechang people＇s hospital from 336 examples carrying out serum to 12 month outpatient service in 2009 and the physical examination centre in July, 2007 mimicking the mark thing checking （HBV-M） diagnosing the hepatitis B patient who is masculine gender, adopt real time fluorescence PCR detecting, carry out analysis on whose result. Result Most liver patients quantify PCR checks the HBV-DNA result and HBV serum mimics the sign thing checking result coinciding. The PCR result copy number is without exception higher patient serum in the group of HBsAg （＋）/HBeAg （＋）/HBeAb （＋） and in the group of HBsAg （＋）/HBeAb （＋）/HBcAb （＋）. HBsAb （＋） or the HBsAg （-） specimen PCR has but individual masculine gender result, whose copy number is very low but. IfHBV copies Conclusion Real time fluorescence PCR detecting HBV-DNA within judging the body, copy the diagnose aspect that degree and HBV serum mimic indicating the feminine gender hepatitis patient, more better than that study of serum indicates thing detecting, is worth being extended clinically.