期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

猪肺炎支原体不同毒力株黏附因子基因序列分析 被引量：9

Sequence Analysis of Adhesin Gene of Different Mycoplasma hyopneumoniae Strains
下载PDF
导出
摘要 将猪肺炎支原体不同毒力株培养获得菌体,提取基因组DNA,PCR扩增黏附因子P97基因,对PCR产物进行测序,将猪肺炎支原体不同毒力株的P97基因序列的碱基组成和推导的氨基酸序列进行比较,分析猪肺炎支原体黏附因子P97基因变化与致病性的关系。结果表明,猪肺炎支原体黏附因子P97基因与菌株致病性有一定关系,但除P97基因之外,还有其他与致病有关的基因存在。 The genomic DNA was extracted from different attenuated Mycoplasma hyopneumoniae strains.The adhesion gene P97 were amplified by PCR,and then were sequenced.The base composition of DNA sequences of P97 and amino acid sequence deduced from different attenuated strains of Mycoplasma hyopneumoniae were compared,and the relationship between genetic variation and virulence was analyzed.The results showed that there was some relationship between genetic variation and virulence of Mycoplasma hyopneumoniae.But there were other genes associated with virulence besides P97.
作者 刘茂军 邵国青 张映
机构地区 江苏省农业科学院兽医研究所 山西农业大学动物科技学院
出处 《江苏农业学报》 CSCD 北大核心 2010年第3期522-525,共4页 Jiangsu Journal of Agricultural Sciences
基金 农业科技成果转化基金项目(2009GB2C100129) 公益性行业(农业)科研专项(200803020) 江苏省农业科技自主创新基金项目[CX(09)118]
关键词 猪肺炎支原体 黏附因子 毒力 序列 Mycoplasma hyopneumoniae adhesion factor virulence sequence
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献17

  • 1HSU T,ARTIUSHIN S,MINION F C.Cloning and functional of the P97 swine cilium adhem gene of Myoplasma hyopneumoniae[J].J Bacteriol,1997,179(4):1317-1323.
  • 2WILTON J L,WALKER M J,DJORDJEVIC S P,et al.Reiterated repeat region variability in the ciliary adhesin gene of Mycoplasma hyopneumoniae[J].Microbiology,1998,144(7):1931-1943.
  • 3萨姆布鲁克 J D,拉塞尔 W.分子克隆实验指南[M].金冬雁,黎孟枫,译.2版.北京:科学出版社,1992.
  • 4OBA T,ANDACH Y,MUTO A,et al.CGG:an unassigned or unsense codon in Mycoplasma capricolum[J].Proc Natl Acad Sci USA,1991,88(3):921-925.
  • 5FUTO S,SETO Y,MITSUSE S,et al.Molecular cloning of a 46-kilodalton surface antigen(P46) gene from Mycoplasma hyopneumoniae direct evidence of CGG condon usage for arginine[J].J Bacteriol,1995,177(7):1915-1917.
  • 6OSAWA S,JUKES T H,WATANABE K,et al.Recent evidence for evolution of the genetic code[J].Microbiol Rev,1992,56(1):229-264.
  • 7DYBVIG K,YU H.Regulation of a restriction and modification system via DNA inversion in Mycoplasma pulmonis[J].Mol Microbiol,1994,12(4):547-560.
  • 8DRITZ S S,CHENGAPPA M M,NELSSEN J L,et al.Growth and microbial flora of nonmedicated,segregated,early weaned pigs from a commercial swine operation[J].J Am Vet Med Assoc,1996,208(5):711-715.
  • 9RAZIN S,YOGEN D,NATO Y.Molecular biology and pathogenicity of Mycoplasmas[J].Mic Mol Bio Rev,1998,62(4):1094-1156.
  • 10吴移谋,叶元康.支原体学[M].北京:人民卫生出版社,2008,16-25.

二级参考文献20

  • 1金冬雁 黎孟枫等（译）.分子克隆实验指南[M].北京:科学出版社,1995..
  • 2蔡宝祥，家畜传染病学（第3版），1996年，141页
  • 3金冬雁（译），分子克隆实验指南（第2版），1995年，16页
  • 4Peterson SN,Bailey CC,Jensen JS,et al.Characterization of repetitive DNA in the Mycoplasma genitalium genome:possible role in the generation of antigenic variation[J].Proc Natl Acad Sci U S A,1995,92(25):11829-11833.
  • 5Fraser CM,Gocayne JD,White O,et al.The minimal gene complement of Mycoplasma genitalium[J].Science,1995,270(5235):397-403.
  • 6Himmelreich R,Hilbert H,Plagens H,et al.Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae[J].Nucleic Acids Res,1996,24 (22):4420-4449.
  • 7Dandekar T,Huynen M,Regula JT,et al.Re-annotating the Mycoplasma pneumoniae genome sequence:adding value,function and reading frames[J].Nucleic Acids Res,2000,28 (17):3278-3288.
  • 8Glass JI,Lefkowitz EJ,Glass JS,et al.The complete sequence of the mucosal pathogen Ureaplasma urealyticum[.J].Nature,2000,407(6805):757-762.
  • 9Sasaki,Y,Ishikawa J,Yamashita A,et al.The complete genomic sequence of Mycoplasma penetrans,an intracellular bacterial pathogen in humans[J].Nucleic Acids Res,2002,30 (23):5293-5300.
  • 10Chambaud I,Heilig R,Ferris S,et al.The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis[J].Nucleic Acids Res,2001,29(10):2145-2153.

共引文献52

同被引文献145

引证文献9

二级引证文献48

江苏农业学报
2010年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部