纳络酮对脑梗死大鼠脑损伤的保护作用及对能量代谢的影响

Protective effects of naloxone on cerebral infarction in rats and its effects on energy metabolism

目的观察纳络酮对脑缺血再灌注大鼠脑损伤的保护作用及对能量代谢的影响。方法采用线栓法建立大鼠局灶性脑缺血-再灌注模型,给予纳络酮后测定大鼠脑梗死面积、神经行为评分、脑含水量;制作脑组织匀浆测定脑组织中丙二醛(MDA)含量、超氧化物岐化酶(SOD)活性,并用HPLC测定脑组织ATP含量和能量负荷值。结果给予纳络酮后大鼠脑梗死面积显著缩小,神经行为障碍显著改善,脑组织水肿得到延缓,丙二醛(MDA)含量显著降低,且脑组织中ATP含量和能荷值显著升高。结论纳络酮对大鼠局灶性脑缺血再灌注损伤有保护作用,其可能机制在于改善了脑组织的能量代谢。

Objective To investigate the protective effects of naloxone on brain injury after cerebral ischemia - reperfusion （ I/ R） and its effects on cerebral energy metabolism. Methods Using middle cerebral ischemic occlusion （MCAO） rats, the cerebral ischemia - reperfusion model was made 3h after ischemia reperfusion. Then, the rats were treated with naloxone. After reperfusion 3 h, 21h, we determined the infarct volume , the extent of water in the brain and the neurological deficit scoring in rats. In the reperfusion 3 h groups, the brain was resected to determine the activity of superoxide dismutase（SOD） ,the content of malonyldialdchyde （MDA）. The high liquid chromatography method was used to detect the contents of ATP, ADP, AMP, and to calculate the energy changes. Resuits In cerebral infarction rats treated with naloxone, the infarct volume was reduced significantly, the neurological deficit score was improved, the extent of water in the brain and content of MDA deceased while the level of ATP and energy change was increased significantly. Conclusions Naloxone has protective effects on brain injury induced by cerebral ischemiarepeffusion. The mechanisms may be related to its improved energy metabolism.