贝前列素钠对兔髂股动脉球囊损伤后内皮细胞的修复作用及对管腔狭窄的影响

Effects of orally active beraprost on rabbit endothelial cell coverage repair and vascular stenosis in the balloon injured iliac-femoral artery

目的评价口服前列环素类似物——贝前列素钠对兔髂股动脉球囊损伤后血管内皮细胞的修复作用及对管腔狭窄的影响。方法将24只雄性新西兰兔随机分为假手术组、模型组和贝前列素钠组,球囊损伤右侧髂股动脉后给予高胆固醇饮食,形成髂股动脉球囊损伤狭窄模型,术后第14天处死实验兔,取出血管标本,经处理后,测定血管内皮细胞覆盖与脱落面积,并测量动脉管腔面积、内弹力层、外弹力层围绕面积,计算内膜、中膜面积和内膜/中膜面积比值。结果 (1)与模型组相比,贝前列素钠组血管内皮细胞覆盖面积较大(P<0.01);(2)贝前列素钠组管腔面积、内弹力层围绕面积和外弹力层围绕面积增加(P<0.05),新生内膜面积减少(P<0.05),内膜/中膜面积比值明显减少(P<0.01)。结论贝前列素钠对兔髂股动脉球囊损伤后内皮细胞的修复有促进作用,可以显著扩张血管和抑制内膜增生,降低血管管腔的狭窄率。

Objective To evaluate the effect of beraprost sodium, an orally active prostacyclin analogue, on rabbit endothelial cell （EC） coverage repair and vascular stenosis in the balloon injured iliac - femoral artery. Methods An iliac - femoral artery steno- sis model was developed in 24 male New Zealand white rabbits by balloon injury and a high - cholesterol diet. At the time of injury, rabbits were randomly assigned to 3 groups： the sham group, the model group and the beraprost group. Rabbits were sacrificed after 14 days and the iliac -femoral arteries were removed and made into pathological sections. The morphological alteration of the artery and the area covered by EC were observed. The internal and external elastic lamina and luminal areas were measured. Then the area of intima vs media was calculated. Results （ 1 ） The EC coverage in the beraprost group obviously increased, compared with the model group （P 〈 0.01 ）. （2） In comparison with the model group, the internal elastic lamina, external elastic lamina and luminal areas in the beraprost group markedly increased （ P 〈 0. 05 ）, the area of intima decreased （ P 〈 0.05 ） and the ratio of intima to media notably decreased （P 〈 0. 01 ）. Conclusions Beraprost can help repair the EC of balloon injured artery in rabbits by dilating the arteries and lessening the neointimal thickness, which result in preventing stenosis in balloon injured arteries in rabbits.