人精子顶体反应的检测及其与穿卵试验的相关性

The determination of the acrosome reaction of human spermatozoa and its relation with zone free penetration test

选择正常生育男子（生育组）、输精管结扎再吻合（吻合组）、不育症患者（不育组）各２５例的精液进行人精子顶体蛋白酶及酸性磷酸酶测定，顶体组织化学三色染色和人精子穿透去透明带金黄仓鼠卵试验，比较不同生育条件下精子功能和生育能力的相关性。结果显示，精子获能后顶体反应率明显高于获能前（Ｐ＜０．０１）。生育组顶体反应率和穿透试验高于吻合组和不育组（Ｐ＜０．０１）。结果表明。

Objective: To determine the acrosome reaction of human spermatozoa and zone free penetration test in fertile, infertile and vasovasotomized men. Methods: Acrosin, sperm acidphosphatase, sperm triple staining and zone free penetration test were performed. The relationship between sperm function and fertility were compared. Results: The results showed that the rate of acrosome reaction was increased obviously after sperm capacitation. The rate of acrosome reaction and penetration test in fertile men were higher than in vasovasotomized and infertile men ( P <0.01). There were statistical differences among the three groups. It suggested that the acrosome reaction is positively related to the penetration capability of sperm ( P < 0.01) Conclusion: The authors consider that combination of routine semen analysis and acrosome reaction tests can provide scientific basis for evaluating human sperm quality in fertilization.