生育与不育男性精子核DNA成熟度的比较研究

Comparitive study of maturity of the sperm nucleus DNA between fertile and infertile men

通过对１９对生育夫妇和７２对不育夫妇丈夫精液中绿色吖淀橙（ＡＯ）荧光精子（成熟精子）比率分布情况的比较分析，探讨了绿色ＡＯ荧光精子百分率与精液参数及精子授精能力之间的关系。在１～３年随访中，通过人工授精、其他治疗或在非治疗期，５４对绿色ＡＯ荧光精子≥５０％的夫妇有１８对怀孕，而１８对绿色ＡＯ荧光精子＜５０％的夫妇无１例怀孕。结果还显示了绿色ＡＯ荧光精子百分率与精液参数无关。认为该项指标从精子核ＤＮＡ成熟状态角度反映了单凭精液分析所不能反映的男性生育力。精液中绿色ＡＯ荧光精子＜５０％。

Objective: To study the relationship of the sperm nucleus DNA maturity and the fertilizing ability of the spermatozoa. Study design: A comparison of distribution percentage of green AO fluorescence sperm (mature sperm) in semen 19 fertile couples and 72 infertile couples. Results: In a follow up survey of 1～3 years after artificial insemination husband (AIH) with or without other therapy, 18 out of 54 infertile couples with green AO fluorescent sperm ≥50% got pregnant, while the other 18 inferile couples with green AO fluorescent sperm <50% were not pregnant. The results indicated that there was no significant relationship between this index and the semen parameters. Conclusions: Index of mature sperm can reflect the fertilizing ability of the spermatozoa on the sperm nucleus DNA maturity state. Green AO fluorescent sperm <50% could be used as an evaluative index for subfertile semen.