利用UP-PCR、ISSR和AFLP标记分析玉米丝黑穗病菌遗传多样性

Genetic diversity of Sporisorium reilianum by UP-PCR,ISSR and AFLP analysis

利用UP-PCR、ISSR和AFLP分子标记方法研究了我国主要玉米产区34株玉米丝黑穗病菌的遗传多样性。从供试引物中筛选获得具多态性的UP-PCR引物9个、ISSR引物11个和AFLP引物组合22对,分别扩增出113、72和293条谱带,多态性条带比率分别为91.15%、84.7%和83.27%。聚类分析表明,玉米丝黑穗病菌存在丰富的遗传变异,与地理来源无明显相关性。3种分子标记的遗传相似系数矩阵相关性分析表明,UP-PCR与AFLP具有较高的相关性,相关系数为0.698;UP-PCR与ISSR、ISSR与AFLP的相关系数分别为0.659和0.633。从多态性水平、稳定性和可操作性可以看出,UP-PCR技术更适于分析玉米丝黑穗病菌遗传多样性。此外,UP-PCR、ISSR和AFLP标记划分的类群与鉴别寄主划分的致病类型之间存在一定的相关性,吻合率分别为50.0%、60.0%和47.6%。

Genetic diversity of 34 isolates of Sporisorium reilianum from main corn producing areas in China was analyzed by UP-PCR （universally primed PCR）, ISSR （internal simple sequence repeat） and AFLP （amplified fragment length polymorphism ）. The results showed that 9 primers of UP-PCR, 11 primers of ISSR and 22 primers of AFLP generated 113, 72 and 293 polymorphic bands, accounted for 91.15% , 84.7% and 83.27% of total bands respectively. These molecular markers revealed the genetic diversity of the strains tested, and there was no significant correlation between variation and region. The correlation between UP-PCR and AFLP, which was 0. 698, was higher than that between UP-PCR and ISSR, or ISSR and AFLP, which were 0. 659 or 0. 633 respectively, by the analysis of genetic similarity coefficient matrix from three kinds of molecular markers. The UP-PCR technique was suitable for the genetic diversity analysis of S. reilianum in view of the level of polymorphism, stability and operability. In addition, there was a certain correlation between the groups divided by UP-PCR, ISSR and AFLP molecular markers and the pathogenic types identified by host identification, coincidence rate were 50.0% , 60.0% , and 47.6% respectively.