新生大鼠缺氧缺血性脑损伤后白细胞介素-1β基因转录的动态观察

A Dynamic Observation on Interleukin 1 β Gene Transcription in Neonate Rats with Hypoxic Ischemic Brain Damage

目的为探讨ＨＩＢＤ发病机制方法制备左脑缺氧缺血性脑损伤（ＨＩＢＤ）的新生大鼠模型，用ＩＬ１β寡核苷酸探针，通过ＤＮＡＲＮＡ斑点杂交技术，检测ＨＩＢＤ后海马、皮质不同时间ＩＬ１β及ｍＲＮＡ。结果斑点杂交技术敏感特异，最低可检出１ｐｇ总ＲＮＡ，且ＩＬ１β寡核苷酸探针只能特异地与其相应核酸杂交。ＨＩＢＤ后１小时ｍＲＮＡ水平开始增高，３小时为高峰表达，１２小时基本消失。其海马表达略高于皮质。结论ＩＬ１β转录水平表达在ＨＩＢＤ后早期出现，其作用可能与ＨＩＢＤ发病过程的炎性过程有关。

Objective To study the change of interleukin 1 β gene transcription in the animal model of hypoxic ischemic brain damage(HIBD).Methods A model of HIBD in left brain of neonate rats was established and IL 1 β mRNA in the lesioned ischemic cortex and hippocampus at different times by DNA RNA Dot blot hybridization with IL 1 β oligonucleotide probe was detected.Results Dot blot hybridization was sensitive and specific.The probe can detect 1pg of total RNA,and hybridize only to the homologous nucleic acid.Level of IL 1 β mRNA was elevated as early as 1 hour and peaking expression was 3 hour after HIBD,IL 1 β expression in hippocampus was higher than that in cortex.Conclusions IL 1 β gene expression appears early after HIBD,it may play an important role in the response of inflammatory to HIBD.