旋复代赭汤促胃肠动力作用的实验研究

Experimental Studies of Stimulative Effect of Xuanfudaizhetang on Gastrointestinal Motility

目的：研究旋复代赭汤对小鼠胃肠动力的作用及其作用机制。方法：通过整体动物实验与离体器官实验相结合，整方实验和拆方实验相结合，多层次综合研究与分析旋复代赭汤的促胃肠动力作用及其作用机制。结果：旋复代赭汤能促进正常状态的小鼠胃排空，对其小肠推进无明显影响；能够拮抗芬氟拉明、左旋麻黄碱、多巴胺引起的小鼠胃排空抑制和小肠推进减慢；对阿托品引起的胃排空抑制有拮抗作用，对其造成的小肠推进减慢无明显影响；不能拮抗吗啡引起的小鼠胃排空抑制和小肠推进减慢。采用正交设计法拆方研究发现，党参、代赭石、大枣和旋复花对大鼠胃底条肌收缩具有显著促进作用，旋复花与甘草、大枣分别合用，有明显协同促进作用。党参、旋复花和半夏能显著拮抗阿托品引起的胃底条肌舒张作用，代赭石和生姜合用，具有协同拮抗作用。结论：旋复代赭汤具有确切的促胃动力作用。

Objective:To study and analyze the stimulative effect of Xuanfudaizhetang(XF) on gastrointestinal motility and the mechanism of this effect.Method: By using in vivo and in vitro experiments with intact and separated recipe.Result: XF markedly facilitated gastric emptying in normal mice,and promoted gastric emptying when gastric motility was delayed by fenfluramine,l ephedrine,dopamine or atropine in mice.XF had no obvious effect on the charcoal meal transit in small intestine of normal mice and the mice treated with atropine,but significantly promoted the delayed small intestinal propulsion induced by fenfluramine,l ephedrine or dopamine.XF had no effect on gastric emptying of small intestinal propulsion in morphine treated mice.The effect of each component of the recipe had been detected by means of orthogonal design,which found that codonopsis pilosula,inula japonica,haematitum and ziziphus jujuba significantly increased the contraction of the isolated strip of rat gastric fundus.A positive synergism on the contraction of the strip of gastric fundus was observed when Inula japonica and glycyrrhiza uralensis or inula japonica and ziziphus jujuba were administered together Codonopsis pilosula,inual japonica and pinellia ternate could antagonize the relaxation induced by atropine in the strip of gastric fundus,showing the synergistic antagonizing effect together with haematitum and zingiber officinale. Conclusion:Xuanfudaizhefang can Promote the motility of gastrointestine profoundly.