白细胞介素18和白细胞介素10参与多囊卵巢综合征发病的初步研究

Study on the correlation between interleukin 18,interleukin 10 and pathogenesis of polycystic ovary syndrome

目的探讨血清炎症因子白细胞介素18(IL-18)、白细胞介素10(IL-10)与多囊卵巢综合征(PCOS)发病的关系。方法选取48例PCOS患者和32例对照,分为肥胖组与非肥胖组。测定血清空腹血糖(FBG)、空腹胰岛素(FINS)、卵泡刺激素(FSH)、黄体生成素(LH)和睾酮(T);酶联免疫吸附试验(ELISA)方法检测血清IL-18、IL-10。结果 PCOS组血清IL-18水平高于对照组,IL-10水平低于对照组,差异有显著性;PCOS肥胖组与非肥胖组相比,IL-18水平升高,IL-10水平下降,差异有显著性;两PCOS组与各自对照组相比,IL-18水平升高,IL-10水平下降,差异有显著性。PCOS组患者血清IL-18与BMI、WHR、FINS及HOMA-IR呈显著正相关,血清IL-10与WHR、FINS及HOMA-IR呈显著负相关。结论 PCOS患者血清中IL-18水平明显升高,IL-10水平明显下降,并且在肥胖的PCOS患者中升高和下降的更明显。慢性炎症可能参与PCOS的发病,并且与胰岛素抵抗和肥胖有关。

Objective：To study the correlation between serum inflammation interleukin 18,interleukin 10 and pathogenesis of polycystic ovary syndrome.Methods：48 cases with PCOS and 32 cases with NO-PCOS were classified as followed：25 obese （BMI≥25kg/m2）and lean（BMI25kg/m2） patients with PCOS;12 obese and 20 lean women without PCOS.Serum levels of fasting glucocose,fasting insulin,follicle-stimulating hormone,luteinizing hormone and testosterone were examined;serum levels of interleukin 18 and interleukin 10 were examined by enzyme-hnked immunosorbent assay.Results：The PCOS group had significantly higher serum IL-18 levels and significantly lower IL-10 levels than those of the control group; The obese PCOS group had significantly higher serum IL-18 levels and significantly lower IL-10 levels than lean group;The two PCOS groups had significantly higher serum IL-18 levels and significantly lower IL-10 levels than their respective controls.Serum levels of IL-18 were positively related to BMI,WHR,FINS and HOMA-IR;and the levels of IL-10 were negtiveli related to BMI,WHR,FIN and HOMA-IR.Conclusion：Serum levels of IL-18 in PCOS group were higher than that in control group,and Sesum levels of IL-10 were lower,especially in obese PCOS patients.Chronic inflammation may participate in the pathogenesisof PCOS,and may have correlation with insulin resistance and obesity.