基质金属蛋白酶13在主动脉夹层患者表达水平的研究

Change of concentration of serum matrix metalloproteinase 13 in patients with aorta dissection

目的探讨主动脉夹层患者血清基质金属蛋白酶13(matrix metalloproteinases,MMP13)表达水平的改变。方法采集经主动脉计算机断层扫描血管造影(CTA)及主动脉造影确诊主动脉夹层患者60例的血液标本,利用酶联免疫吸附测定方法(ELISA)测定血清MMP13浓度;同时测定30例年龄、性别相匹配健康者的血清MMP13浓度。结果主动脉夹层患者的血清MMP13浓度较正常对照组升高,差异有统计学意义[(41.94±4.34)ng/mLvs.(20.20±1.86)ng/mL,P<0.01]。血压控制理想组和血压控制不理想组的血清MMP13浓度略高于血压正常组,但差异无统计学意义(P>0.05)。血压控制理想组的血清MMP13浓度反而略高于血压控制不理想组,而差异无统计学意义(P>0.05)。另外,原发性高血压(高血压)病程大于5年组的血清MMP13浓度虽然略高于病程小于5年组,但是差异亦无统计学意义(P>0.05)。结论血清MMP13浓度升高可能为主动脉夹层发病的原因之一,且与高血压病程及血压控制情况无关。

Objectives To investigate the change of concentration of serum matrix metalloprotenase 13 （MMP13） in patients with aorta dissection. Methods Blood samples of 60 patients who were confirmed as aorta dissection by aortic computed temography angiography（CTA） and angiography, were collected. Concentrations of serum MMP13 of all the collected samples were measured by enzyme-linked immunosorbent assay （ELISA）, and compared with 30 healthy controls （with consistent age and gender）. Results Concentrations of serum MMP13 of aorta dissection patients were significantly higher than that of normal control [（41.94 ±4.34）ng/mL vs. （20.20 ±1.86）ng/mL,P〈0.01]. Concentrations of serum MMP13 of blood pressure controlled group and uncontrolled group were slightly higher than that of normal blood pressure group with no significant difference （P〉0.05）. And concentrations of serum MMP13 of blood pressure controlled group were slightly higher than uncontrolled group with no significant difference （P〉0.05）. Concentrations of MMP13 in longer than 5 years course of hypertention group were slightly higher than that of the shorter than 5 years group with no significant difference （P〉0.05）. Condusions The elevation of serum MMP13 may be the possible cause of aorta dissection and was also independent of hypertension.