内镜检查前检测HBV血清标志物及HBV DNA的意义

The meaning of inspecting HBV serum indicator and HBV DNA before endoscopic detection

目的探讨内镜检查的安全性和预防医院感染,评估人群HBV血清标志物及HBVDNA的感染率。方法采用免疫吸附试验(ELISA)和荧光定量(PCR)技术分别检测需要做内镜检查才能确诊的患者和初筛合格征兵对象的标本进行HBV血清标志物及HBVDNA的检测。结果经ELISA法检测,346例患者中乙型肝炎病毒标志物阴性62份,其中呈HBVDNA阳性1例(1.6%);在300份初筛合格的征兵对象标本中,6例呈HBVDNA阳性(2%),其中222份ELISA法全阴性的标本检出HBVDNA阳性2例(0.9%)。结论所检测标本中有HBsAg阴性的HBV感染;可见人群中存在HBsAg阴性的HBV感染,乙型肝炎病毒DNA和血清标志物的联合检测,对预防内镜检查感染和提高征兵对象的身体素质有重要的意义。

[Objective]Discuss the safety of Endoscopic detection and prevention of hospital infection, evaluate public HVB serum indicator and the ratio of HBV-DNA infection.[Method]HBV serum indicator and HBV-DNA sample were detected by ELISA and PCR for the patients who only can be diagnosed after endoscopic detection and qualified recruitment target of first filtration. [Result]Through ELISA determination, 62 case hepatitis B virus indicators were negative and 1case （1.6%） HBV-DNA was masculine from total 346 patients; from 300 samples of qualified recruitment target of first filtration, 6 cases （2% ） HBV-DNA were masculine, 222 complete negative samples by ELISA, in which 2 cases （0.9%） HBV DNA were masculine. [Conclusion]There are HBsAg negative HVB infection in the detected samples; it means there is HbsAg negative HVB infection in public, so hepatitis B virus DNA detection is associated with serum indicator, it is significant to preventing endoscopic detection infection and improving recruitment target body quality.