UPLC同时测定荭草药材中8种指标成分的含量

Simultaneous determination of eight components in Polygonum orientale by ultra performance liquid chromatography (UPLC) method

目的:本研究采用UPLC建立了同时测定贵州产荭草药材中儿茶素、异荭草素、荭草素、槲皮素-3-O-(2″-O-α-鼠李糖)-β-葡萄糖醛酸、花旗松素、木犀草苷、槲皮苷、山柰素-3-O-β-D-葡萄糖苷8种指标成分的含量。方法:ACQU-ITY UPLC BEHC18色谱柱(2.1 mm×150 mm,1.7μm),乙腈-0.1%磷酸水溶液为流动相,流速为0.30 mL.min-1,梯度洗脱,检测波长260 nm,柱温45℃。试验分别对线性、仪器精密度、重复性、中间精密度、准确性等项目进行了验证。结果:被测定的8个化合物分别在选定的范围内线性关系良好(r≥0.999 8),平均加样回收率在97.6%～102.5%,RSD 1.9%～4.7%(n=6)。结论:贵州产荭草的化学成分基本相同,但被测定的成分含量有一定差别,在8月份采收的药材中被测成分的含量较高且较为稳定。

In this study,a UPLC-PDA method for simultaneous determination of catechin（1）,isoorientin（2）,orientin（3）,quercetin-3-O-（2″-O-α-rhamnopyranosyl）-β-glucarono-pyranoside（4）,taxifoliol（5）,luteolin（6）,quercitrin（7） and kaempferol-3-O-β-D-glucoside（8） in Polygonum orientale in Guizhou province was developed.Analysis was performed at 45 ℃,on ACQUITY UPLC BEH C18 column（2.1 mm × 150 mm,1.7 μm）,eluted with a gradient program of acetonitrile-0.1% H3PO4 aqueous solution as the mobile phases.The flow rate was of 0.30 mL·min-1,and the detection wavelength was 260 nm.The method validation proved that the linearity,instrument precision,repeatability,intermediate precision and accuracy accorded with the requirement for anassay.The established method had been used for determination above eight components in twenty lots of P.orientale in different places and harvest time in Guizhou province,and the results showed that the chemical composition was basically the same in herbs,but there were some difference in content of the tested components.the contents of the tested ingredients were relatively high and stable in the sample collected in August.