GC-MS法分析蚕蛾油与蚕蛹油的脂肪酸组成

GC-MS Analysis of Fatty Acid Composition of Silkworm Male Moths and Pupa

采用超临界CO2萃取雄蚕蛾与蚕蛹中的油脂,经甲酯化处理用气相色谱-质谱联用仪进行分析,在雄蚕蛾油中分离鉴定出15种脂肪酸,含量最高的是α-亚麻酸(36.71%),其中直链饱和脂肪酸(SFA)54.52%;单不饱和脂肪酸(MUFA)6.89%;多不饱和脂肪酸(PUFA)38.63%。在蚕蛹油中共分离鉴定出14种脂肪酸成分,含量最高的是亚油酸(39.03%),其次是α-亚麻酸(33.22%),其中直链饱和脂肪酸(SFA)占25.73%;单不饱和脂肪酸(MUFA)占2.12%;多不饱和脂肪酸(PUFA)占72.25%。

In this study,we used GC-MS to analyze the fatty acid composition of essential oils extracted from silkworm male moths and pupa with supercritical CO2 after methylation.Fifteen fatty acids were separated and identified in silkworm male moths oil,containing 54.52% of saturated fatty acids（SFA） and 6.89% of mono-unsaturated fatty acids（MUFA） and 38.63% of polyunsaturated fatty acids（PUFA）,among whichs alpha linlenic acid presented the highest relative content（up to 36.71%）.Fourteen fatty acids were identified in silkworm pupa oil,among which,the relative content of linoleic acid was the highest（up to 39.03%）,followed by alpha linlenic acid（33.22%）;SFA,MUFA and PUFA accounted for 25.73%,2.12% and 72.25%.