光皮树籽抽出物的成分分析

GC-MS Analysis of Fatty Acid Composition of Different Organic Solvent Extracts from Cornus wilsoniana Seeds

为了分析光皮树籽抽提物的化学成分,采用无水乙醇、丙酮、正己烷、石油醚和乙酸乙酯分别抽提光皮树籽,抽出物经活性炭脱色后进行GC-MS分析。结果发现,无水乙醇和丙酮抽出物中主要含有游离的棕榈酸、油酸和亚油酸,其中亚油酸相对含量最高,达到52%以上,油酸相对含量最低,不超过8.2%。正己烷、石油醚和乙酸乙酯抽出物中甘三酯的脂肪酸组成主要是棕榈酸、硬脂酸、油酸和亚油酸,其中油酸相对含量最高,达到42%以上,其次是亚油酸,总不饱和脂肪酸相对含量超过70%。抽出物中未发现三不饱和脂肪酸。

Cornus wilsoniana seeds were extracted with absolute ethanol,acetone,hexane,petroleum ether and ethyl acetate respectively,and the extracts obtained were decolorized with active carbon prior to GC-MS analysis.Palmitinic acid,oleic acid and linoleic acid were the predominant compounds in ethanol and acetone extracts.Among them,the relative content of linoleic acid was the highest,as high as more than 52% and that of oleic acid was the lowest,less than 8.2%.The main compounds in respective methyl esterification products of hexane,petroleum ether and ethyl acetate extracts were palmitinic acid,steric acid,oleic acid and linoleic acid.Among them,the relative content of oleic acid was the highest,as high as more than 42%,followed by linoleic acid.The relative contents of total unsaturated fatty acids in hexane,petroleum ether and ethyl acetate extracts were all more than 70%.No triunsaturated fatty acids were detected in any of the 5 extracts.