液质法测定猪肉中八种糖皮质激素残留

Determination of Glucocorticoid Residues in Pork by HPLC-MS/MS

建立猪肉中糖皮质激素多残留的液相色谱串联质谱(LC-MS/MS)分析方法。样品经甲醇提取,固相萃取柱净化后采用LC-MS/MS进行检测分析。对样品前处理条件和质谱参数进行研究,这些激素的物质的线性范围均为0.5～100μg/kg。低、中、高3种质量浓度加标回收率均为78%～101%;最低检出限为0.2～2μg/kg。该方法快速简便,灵敏度高,选择性好,测定结果令人满意,可在各食品检测机构推广应用。

A sensitive and rapid method using HPLC-MS/MS was proposed for the determination of 8 glucocorticoid residues in pork.Samples were extracted with methanol and cleaned up on a solid-phase extraction column before HPLC-MS/MS analysis.Optimum sample pretreatment conditions and mass spectral parameters were investigated.Calibration curves for 8 glucocorticoids showed good linearity over the concentration range from 0.5 to100μg/kg.Average recoveries for them spiked at 3 levels were between 78% and 101%.The method developed exhibited a limit of detection for all the analytes ranging from 0.2 to 2μg/kg.The determination of real samples was carried out using this method and satisfying results were obtained.This method is simple,rapid,sensitive and highly selective and is worth popularizing among food inspection organizations.