噻莱昔布对体外钛微粒诱导大鼠成骨细胞凋亡的影响

In vitro effect of celecoxib on osteoblast apoptosis induced by titanium particle in neonatal rats

[目的]探讨噻莱昔布对Ti微粒诱导体外培养的新生大鼠成骨细胞凋亡的影响。[方法]取体外培养的新生大鼠成骨细胞,MTT法测得促成骨细胞增殖的最佳含药血清浓度。分别加入最佳浓度的含生理盐水血清的Ti微粒和含噻莱昔布血清的Ti微粒混合培养。24h后收集细胞液,采用酶联免疫吸附法检测成骨细胞TNF-α、PGE-2的浓度;实时荧光定量PCR法检测COX-2mRNA表达;WESTREN BLOT检测COX-2蛋白的表达。[结果]Ti微粒可诱导成骨细胞COX-2 mRNA表达,进而刺激TNF-d、PGE-2等炎症因子释放;噻莱昔布可明显抑制COX-2 mRNA的表达。[结论]噻莱昔布可或许可以用来防治磨损微粒引起的人工关节松动的发生。

[ Objective] To study the effect of celecoxib on osteoblast apoptosis induced by titanium particle in neonatal rats. [ Methods] The best in vitro eelecoxib serum concentration for promoting proliferation of osteoblast in neonatal rats was obtained through MTT. One group of rat osteoblast in vitro was cultured with serum containing saline and titanium particle and the other was cultured with serum containing celeeoxib and titanium particles. Concentrations of TNF - α and PGE - 2 in cells col- lected after 24 hours were analyzed by ELLSIA, COX - 2 mRNA and protein were indicated by FO - PCR and Westren blot. [ ResnitsJ Some cellar factors such as COX-2, TNF-α and PGE-2 could be induced by titanium particle in in vitro, but celeeoxib could inhibit expression of these factors by COX -2 pathway. [ Conclusion] Celeeoxib coule be a promising drug for treatment of aseptic loosening.