大鼠部分肝移植模型建立的技术要点

TECHNICAL IMPROVEMENT OF PARTIAL LIVER TRANSPLANTATION IN RATS

目的为提高大鼠部分肝移植模型的稳定性,探讨手术操作的要点。方法采用二袖套法进行大鼠全肝移植(OLT)、50%部分肝移植(50%PLT)和30%部分肝移植(30%PLT),分析手术和存活状况。结果 OLT组、50%PLT组和30%PLT组肝移植大鼠术后一周存活率分别为91.7%、66.7%和8.3%,差别显著(P<0.01)。部分肝移植大鼠的死亡原因有小体积综合征引起的原发性移植肝无功、肺部感染、肝上下腔静脉吻合口扭曲成角、肝下下腔静脉栓塞和门静脉栓塞。结论:减少游离肝脏的机械损伤、细致的供肝修整、提高吻合口质量、缩短无肝期和下腔静脉阻断时间、注重受体大鼠体温的恢复是部分肝移植模型成功的关键。

Objective To investigate how to establish a stable model of partial liver transplantation in rats by improving technical skills.Methods Orthotopic liver transplantation （OLT）50% partial liver transplantation （50%PLT） and 30% partial liver transplantation （30%PLT） were performed according to two-cuff technique.Operative parameters and survival rates were observed and analyzed.Results One week survival rate of OLT,50%PLT and 30%PLT were 91.7%,66.7% and 8.3% respectively,with significant statistical difference （P〈0.01）.Causes of death in partial liver transplantation were primary graft nonfunction induced by small-for-size syndrome,pulmonary infection,distortion of the anastomosis of suprahepatic vena cava,thrombosis of infrahepatic vena cava and portal vein.Conclusion Lessening the mechanical injury in liver procurement,meticulously back-table operating of hepatic graft,improving the anastomosis quality,reducing the anhepatic phase and the vena cava clamping period,and restoring the recipient body temperature would be the key points to successful establishment of partial liver transplantation model in rats.