人类表皮生长因子受体-2高亲和力模拟肽对卵巢癌细胞SKOV3生长的抑制作用

Effect of peptide mimic on ovarian cancer cell line in vitro

目的 研究人类表皮生长因子受体-2（Her-2）高亲和力模拟肽（YCFPDEEGACY）对卵巢癌细胞株SKOV3增殖的影响.方法 化学合成模拟多肽,用MTT法检测合成多肽对SKOV3细胞株体外生长的抑制作用.结果 含模拟肽成分组与阴性对照组比较（t≥2.896,P〈0.05） 单纯EGF组与相应EGF和模拟肽共同作用组比较（f≥3.355,P〈0.05）,显示模拟肽对Her-2高表达的卵巢癌细胞株SKOV3的体外生长有明显抑制作用,对Her-2低表达的乳腺癌细胞株MDA-MB-231无明显抑制作用.结论 Her-2高亲和力模拟多肽可与Her-2高表达的卵巢癌细胞特异结合并能明显抑制其在体外的生长,为卵巢癌靶向生物治疗提供依据.

Objective To study the inhibition of peptide mimic（YCFPDEEGACY）on ovarian cancer cell line positive for Her-2 expression.Methods Peptide mimic was synthesized through chemical ways,and the inhibition effect of peptide mimic on SKOV3,MDA-MB-231 cell lines expressing Her-2 were evaluated with MTr assay. Results Between mimic peptide groups and negative control,there Was statistical significance in mean of A490（t≥2.896,P〈0.05）.Different dose EGF group compared with its added mimic peptide group,there was also statistical significance in mean of A490（t≥13.355,P〈0.05）.Cell proliferation assay showed that peptide mimic could inhibit cell proliferation of SKOV3. Conclusion Peptide can specifically bind to ovarian cancer cell line SKOV3 that overexpress Her-2 and inhibit its proliferation.