结肠癌中Wnt/β-catenin信号通路与FAK关联性的初步研究

Initial study of the relationship between Wnt/β-catenin signaling and FAK in colorectal carcinoma

目的研究β-连接素(β-catenin)和黏着斑激酶(FAK)在结肠癌中的表达及其临床病理参数的关系,探讨Wnt/β-catenin信号通路和FAK的关联性。方法收集60例结肠癌组织标本和8例距病灶3～5cm的癌旁正常肠黏膜组织标本,采用免疫组织化学技术检测两类标本中β-catenin和FAK的表达水平,将与其临床病理资料相结合进行综合分析。结果正常β-catenin、异常β-catenin和FAK在结肠癌中的阳性表达率分别为66.7%(40/60)、63.3%(38/60)和73.3%(44/60),β-catenin表达和FAK的阳性表达与肿瘤的侵袭、转移密切相关,而与患者的年龄、性别和肿瘤的大小及分化类型均无关。β-catenin异常表达和FAK之间呈明显正相关(r=0.487,P=0.000)。结论β-catenin和FAK的表达与结肠癌的侵袭、转移和预后密切相关,Wnt/β-catenin信号通路和FAK之间存在正相关。

[Objective]To explore the relationship between Wnt/β-catenin signaling and FAK by detecting of the expression of β-catenin and FAK in colorectal carcinoma. [Methods]Immunobhistochemistry was used to detect the expression of β-catenin and FAK protein in 60 specimens of colorectal carcinoma and 8 specimens of normal colorectal tissues. The corresponding clinical data were analyzed retrospectively. [Results]The positive rate of normal β-catenin, abnormal β-catenin and FAK expression in colorectal carcinoma was 66.7%, 63.3% and 73.3%, respectively. β-catenin and FAK expression were significantly correlated with the depth of invasion and lymph node metastasis, but not with the age, sex, and the tumor size or position and differentiation of the patients. There existed positive correlation between the expression of abnormal expression of β-catenin and FAK （r =0.487, P =0.000）. [Conclusion]The expression of β-catenin and FAK are closely correlated with the invasion/metastasis and prognosis of colorectal carcinoma. There maybe existed relationship between Wnt/β-catenin signaling and FAK in colorectal carcinoma.