虾夷扇贝中大肠杆菌的累积及净化技术研究

Accumulation and depuration of Escherichia coli in scallop Patinopecten yessoensis

研究大肠杆菌在扇贝体内累积及组织分布的规律。将污染后的虾夷扇贝放入经臭氧系统灭菌后的海水中,净化处理,以大肠菌群为指标,探讨影响虾夷扇贝净化效果的几个关键因素,并对净化前后虾夷扇贝的营养成分进行检测。结果表明,虾夷扇贝各组织中大肠菌群浓度由高到低依次为:消化腺>性腺>腮和外套膜>闭壳肌。当臭氧浓度为0.03mg/kg时,最佳净化工艺条件为,贝水比1:8(m:m);盐度3.3%;净化时间为32h。净化前后,虾夷扇贝中各营养成分含量并无明显变化。试验证明,臭氧净化法能清除虾夷扇贝的微生物污染,且不影响扇贝的营养成分。

After exposure to Escherichia coli,the accumulation and tissue distribution were studied of E.coli in Patinopecten yessoensis.Meanwhile,taking the E.coli level as a parameter index,an experiment was designed to determine several key factors influencing the aseptic sea water depuration of Patinopecten yessoensis by ozone.Furthermore,the changes in nutrient composition in Patinopecten yessoensis were assayed during the depuration.The result showed that the level of E.coli forms throughout the tissues of the Patinopecten yessoensis exhibited the following orders：digestive tract〉gonad〉gills and labial palps〉muscles.The optimal depuration condition was obtained as follows：scallop weight to water weight is 1：8（m：m）,salinity is 33,depuration time is 32 h.It indicated that the nutritious constituents of the Patinopecten yessoensis samples were hardly influenced by the depuration process.This study demonstrated the ozone depuration method could not only reduce Patinopecten yessoensis microbiological contaminations,but also could retain its nutrition.