老年男性甲状旁腺激素基因多态性与骨密度的相关性研究

Association between gene polymorphisms of parathyroid hormone and bone mineral density in eldly men

目的:探讨甲状旁腺素(PTH)基因多态性与中国广东地区部分汉族男性人群骨密度的相关性。方法:随机筛选年龄65～86岁,(70.09±6.69)岁,广州男性264例,采用双能X线吸收法测其全身、腰椎2～4、股骨颈、Ward′三角和大转子区等部位的骨密度(BMD)值,并采用聚合酶链反应限制性片段长度多态性方法检测外周血白细胞基因组PTH基因型。结果:264例受试对象中,PTH基因BB型181例(68.6%)、Bb型73例(27.6%);和bb型10例(3.8%)。PTH基因BstBI基因型分布符合Hardy-Wenbeng平衡定律。三种不同的基因型相互之间的骨密度均无统计学意义(P>0.05)。结论:探讨甲状旁腺素(PTH)基因多态性与广东地区汉族老年男性人群骨密度关系不密切,不能作为筛查和预示骨质疏松症的遗传易感位点。

Objective To Investigate the association between gene polymorphisms of parathyroid hormone （PTH） and bone mineral density （BMD） in eldly men in Guangzhou. Methods Data from 264 eldly men in Guangzhou [aged 65 ～ 86 years, mean （70.09±6.69 ）years] were included. The genotypes of PTH gene were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RELP）, and the BMD of lumbar, inter, trach, vertebrae, femur neck, and Ward′ s triangle was measured by dual-energy X-ray absorptiometry （DEXA）. Results The distribution of PTH gene genotypes were as following： BB genotype in 181 cases （68.6%）, Bb in 73 cases （27.6%）, and bb in 10 cases （3.8%）, and were consistent with the Hardy- Weinberg equilibrium. There were no significant differences of BMD among these genotype groups （P 0.05）. Conclusion There is no obvious correlation between the gene polymorphisms of PTH and BMD in eldly men in Guangzhou. PTH genotype may not be used as genetic marker in predicting the risk of osteoporosis.