非小细胞肺癌与血清中2种相关因子含量关系

Relationship between contents of two correlative factors in serum and non-small cell lung cancer

目的探讨非小细胞肺癌(NSCLC)患者血清中血管内皮细胞生长因子(VEGF)和基质金属蛋白酶-9(MMP-9)与其病理类型和Primary Tumor-RegionalLymph Node-Distant Metastasis分期(TNM分期)的关系。方法选取NSCLC患者97例,采用酶联免疫吸附法(ELISA)检测其血清中MMP-9和VEGF含量,采用t检验、方差分析及秩相关分析对不同病理类型和不同TNM分期的2种因子检测结果进行比较。结果 50例腺癌及47例鳞癌患者血清中VEGF含量差异无统计学意义(P>0.05);Ⅰ、Ⅱ、Ⅲ、Ⅳ期肺癌患者血清中VEGF含量分别为(251.1±72.43),(261.30±67.21),(279.1±74.67),(313.2±75.23)pg/mL,差异有统计学意义(F=11.12,P<0.01);TNM分期与血清中VEGF含量呈正相关(r=0.62,P=0.01);腺癌与鳞癌患者血清中MMP-9含量分别为(856.7±81.5),(623.2±75.2)ng/mL,差异有统计学意义(t=2.54,P<0.05);Ⅰ、Ⅱ、Ⅲ、Ⅳ期肺癌患者血清中MMP-9含量分别为(602.8±78.4),(714.5±79.3),(880.5±72.9),(934.5±73.8)ng/mL,差异有统计学意义(F=9.00,P=0.01),TNM分期与血清中MMP-9含量呈正相关(r=0.52,P=0.01);非小细胞肺癌患者血清中2种因子呈高度正相关关系(r=0.74,P<0.01)。结论 NSCLC患者血清中MMP-9和VEGF含量与TNM分期关系密切,2种因子间呈明显协同作用。

Objective To explore clinical significance of serum vascular endothelial growth factor（VEGF）,matrix metalloproteinase-9（MMP-9） and its relationship with the TNM staging and pathological types of non-small cell lung cancer（NSCLC）.Methods The contents of serum VEGF and MMP-9 were measured with ELISA in 97 patients with different TNM staging of NSCLC.The t-test,ANOVA and rank correlation coefficient were used for data analysis.Results The levels of serum VEGF between 50 patients with lung glandular carcinoma and 47 patients with lung squamous cell carcinoma was not statistically different（P 0.05）.The levels of serum VEGF of Ⅰ,Ⅱ,Ⅲ and Ⅳ in lung cancer patients were 251.1 ± 72.43,261.30 ± 67.21,27.91 ± 74.67,313.2 ± 75.23 pg /ml,respectively,with significant difference（F = 11.12,P 0.01）.The TNM staging and the levels serum VEGF in NSCLC were positive correlative（r = 0.62,P = 0.01）.The levels of serum MMP-9 of patients with lung glandular carcinoma and patients with lung squamous cell carcinoma were 856.7 ± 81.5 and 623.2 ± 72.2 ng /ml with significant difference（t = 2.54,P 0.05）.The levels of serum MMP-9 of Ⅰ,Ⅱ,Ⅲ and Ⅳ in lung cancer patients were 602.8 ±78.4,714.5 ±79.3,880.5 ±72.9,and 934.5 ±73.8 ng/ml with significant difference（F = 9.00,P = 0.01）.The TNM staging and the levels serum MMP-9 in NSCLC were positive correlative（r =0.52,P =0.01）.The two correlative factors in serum of patients with NSCLC were positive correlative（r = 0.74,P 0.01）.Conclusion The levels of serum VEGF adn MMP-9 in patients with NSCLC are closely correlated with TNM staging and there is a synergic effect between the two correlative factor.