镉对雄性大鼠组织中镍残留影响

Effect of CdCl_2 on tissue NiCl_2 residue in rats

目的应用同位素示踪法,研究镍在大鼠体内的药物动力学参数及镉对镍在大鼠组织内残留的影响。方法选取36只大鼠,随机分为6组,1组镍镉混合液灌胃给药后,不同时间断尾取血,另5组不同剂量镍镉混合液灌胃15d后处死,血样和各组织消化处理后测量放射性计数。结果镍的血液浓度-时间曲线符合二房室开放模型,0.53h达到吸收峰值24987.75cpm/mL,随后很快消除,清除速率常数为0.42mL/(kg·h);镉剂量为0mg/kg时,镍在所有组织中均有残留;镉剂量较低时(0.5,1,10mg/kg),几乎所有组织均未发现组织残留;当镉的剂量升高到25mg/kg,镍残留量有所回升,但仍低于单独给镍时;当镉剂量为10mg/kg时,眼球、肾脏和毛中镍残留量分别为(0.72±0.31),(0.00±0.01),(1.58±0.78)kBq,与其他组织比较,差异有统计学意义(P<0.05)。结论镉对镍在大鼠各器官内残留量影响较大,小剂量镉明显抑制镍的组织残留,剂量较大时,抑制效应有所减弱。

Objective To studty the effect of cadmium on tissue residue of nickel and parameter of pharmacokinetics of nickel isotope tracer in rats.Methods The rats in six groups were intragastrically administrated with mixture of 63NiCl2 and different doses of CdCl2,respectively.Fifteen days after the administration,the rats were sacrificed and their tail blood and tissue samples were collected.The radioactivity of the samples were detected with liquid scintillation counting.Results The concentration-time curves of nickel in the blood fitted the two-dicompartment model of pharmacokinetics.After the rats were treated with single dose of nickel without cadmium,residue of nickel in almost all tisssues were higher.After the rats were exposed to both lower dose cadmium and nickle,residue of nickel in all tissues was lower.After the rats were exposed to both higher dose of cadmium（25 mg /kg） and nickel,residue of nickel in tissues was increased again.Conclusion The obvious influence of cadmium on nickel residue in rats was found.The low dose of cadmium can inhibit absorption of nickel and the inhibition is weak for high dose of cadmium.