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黄铜矿表面生物吸附量的测定条件 被引量:3

Conditions for Biosorption Measuring on Chalcopyrite Surface
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摘要 采用茚三酮比色法测定了在细菌浸矿的过程中,黄铜矿矿物表面吸附细菌细胞裂解液的吸光度,通过对测量波长、裂解时间、溶液pH值、加热时间、冷却时间等影响吸光度因素的实验研究,得出了黄铜矿表面细菌细胞裂解液吸光度的最佳测定条件为:波长560nm,裂解时间25min,溶液pH值7.0,加热时间15min,冷却时间6min.在最佳条件下绘制出了吸光度与矿物表面细菌吸附量之间关系的标准曲线.研究表明细菌裂解液的吸光值与细菌的浓度成正比. The ninhydrin colorimetry was used to measure the absorbance of the bacterial cell lysis solution adsorbed on the surface of chalcopyrite during bacterial leaching. The influencing factors on the absorbance, such as measuring wavelength, lysing time, pH value of the solution, heating time and cooling time were investigated experimentally to get the optimal measuring conditions for the absorbance of the lysis solution, i.e. , wavelength 560 nm, lysing time 25 min, pH value 7. 0, heating time 15 min and cooling time 6 min. A standard curve showing the relationship between the absorbance and bacteria adsorption under optimal conditions was therefore given. It was found that the bacterial concentration is directly proportional to the absorbance of the lysis solution.
出处 《东北大学学报(自然科学版)》 EI CAS CSCD 北大核心 2010年第7期999-1002,共4页 Journal of Northeastern University(Natural Science)
基金 国家自然科学基金资助项目(50674029 50874030) 国家高技术研究发展计划项目(2006AA06Z127) 教育部高等学校博士学科点专项科研基金资助项目(20060145015) 国家科技支撑计划项目(2008BAB34B01)
关键词 生物吸附 茚三酮比色法 黄铜矿 细菌 矿物表面 biosorption ninhydrin colorimetry chalcopyrite bacteria mineral surface
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  • 1Schaeffer W I, Holbert P E, Umbreit W W. Attachment of thiobacillus thiooxidans to sulfur crystals [ J ]. Journal Bacteriology, 1963,85 : 137 - 140.
  • 2Free M L, Oolman T, Nagpal, S, et al. Bioleaching of sulfide ores distinguishing between indirect and direct mechanism[J]. Mineral Bio-processing, 1991,7: 76 - 89.
  • 3Murthy K S N, Natarajan K A. The role of surface attachment of thiobacillus ferooxidans on the bicoxidation of pyrite[J]. Minerals & Metallurgical Processing, 1992,2: 24 29.
  • 4Ohmura N, Tsugita K, Koizumi J, et al. Sulfur-binding protein of flagella of thiobaeillus ferrcoxidans[J]. Journal of Bacteriology, 1996,178 (19) : 5776 - 5780.
  • 5Sampson M I, Blake II R C. Cell attachment and oxygen consumption of two strains of thiobaeillus ferrcoxidans [ J ]. Minerals Engineering, 1999,12(6) :671 - 686.
  • 6Sampson M I, Phillips C V, Blake II R C. Influence of the attachment of acidophilic bacteria during the oxidation of mineral sulfides [ J ]. Minerals Engineering, 2000, 13 (4) : 373 - 389.
  • 7Sampan M I, Blake II R C. Cell attachment and oxygen consumption of two strains of thiobacillus ferrooxidans [J]. Minerals Engineering, 1999,12(6) :671 - 686.
  • 8Escobar B, Jedlicki E, Wiertz J, et al. A method for evaluating the proportion of free and attached bacteria in bioleaching of cbaleopyrite with thiobaeillus ferrooxidans[J ]. Hydrometallurgy, 1996,40(1/2) : 1 - 10.
  • 9Karan G, Natarjan K A, Modak J M. Estimation of mineral -adhered biomass of thiobacillus ferrooxidans by protein assay-some problems and remedies[J ]. Hydrometallurgy, 1996,42(2) :169 - 175.
  • 10周吉奎,钮因健,邱冠周,覃文庆.茚三酮比色法测定矿物表面吸附浸矿细菌蛋白质含量[J].中南工业大学学报,2003,34(2):128-131. 被引量:12

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  • 1朱长见,陆建军,陆现彩,王汝成,李奇.氧化亚铁硫杆菌作用下形成的黄钾铁矾的SEM研究[J].高校地质学报,2005,11(2):234-238. 被引量:49
  • 2杨少华,杨凤丽,余新阳.难处理金矿石的细菌氧化机理及影响因素[J].湿法冶金,2006,25(2):57-60. 被引量:11
  • 3郑志宏,吴为荣,李寻,刘亚洁,张卫民.耐低pH氧化亚铁硫杆菌的培养与驯化[J].有色金属(冶炼部分),2007(4):30-32. 被引量:4
  • 4杨显万,沈庆峰,郭玉霞.微生物湿法冶金[M].北京:冶金工业出版社,2008:25.
  • 5Jensen A B, Webb C. Ferrous sulphate oxidation using thiobacillus ferrooxidans : a review[J]. Process Biochemistry, 1995,30 (3) : 225 - 236.
  • 6Brierley J A, Brierley C L. Present and future commercial applications of biohydrometallurgy [ J ]. Hydrometallurgy, 2001,59(4):233 239.
  • 7Brierley J A. Response of microbial systems to thermal stress in biooxidation heap pretreatment of refractory gold ores[ J ]. Hydrometallurgy, 2003,71(1/2) : 13 - 19.
  • 8Bennett J W, Ritchie A I M. A proposed technique for measuring in situ the oxidation rate in biooxidation and bioleaeh heaps[J]. Hydrometallurgy, 2004,72 ( 1/2) : 51 - 57.
  • 9Rawlings D E. Biomining: theory microbes and industrial processes[M]. Berlin: Springer, 1997 : 117 - 127.
  • 10Harver T J, Merwe W V, Afewu K. The application of the GeoBiotics GEOCOAT" bio-oxidation technology for the treatment of sphalerite at Kumba Resources' Rosh Pinah Mine[J]. Minerals Engineering, 2002,11 (15) : 823 - 829.

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