日粮中添加高水平纳米硒对蛋鸡血硒含量及抗氧化能力的影响

Effects of High Levels of Nano-Se on Blood Selenium Content and Antioxidant Abilities in Hens

选取288只42周龄健康农大3号商品蛋鸡,随机分为6个组,设4个重复,在基础日粮中以亚硒酸钠的形式添加硒0.3mg/kg为对照组;其余各组以纳米硒的形式分别添加硒0.3、0.5、2.5、5.0mg/kg和10.0mg/kg。试验期为60d。结果表明:日粮中添加2.5～10.0mg/kg纳米硒能显著提高全血硒的含量(P<0.05)。试验至30d,日粮中添加10.0mg/kg纳米硒显著提高血浆T-AOC活性;日粮添加0.3～10.0mg/kg纳米硒能显著降低血浆MDA和血浆NO含量(P<0.05)。试验至60d,添加5、10.0mg/kg纳米硒能显著降低GSH-Px活性,添加2.5～10.0mg/kg显著提高NO含量(P<0.05)。综上所述,蛋鸡日粮中添加纳米硒至2.5mg/kg是安全的。

The Experiment was conducted to investigate effects of the different level nano-selenium on blood selenium content and antioxidant abilities in hens. Two hundred and eighty eight 42-weeks-old healthy Nong Da 3 hens were randomly divided into six groups, with 48 each group. The hens of the control group were fed the diet adding selenium 0.3 mg/kg as Na2SeO3. The hens in other groups were fed the diet adding selenium 0.3, 0.5, 2.5, 5.0 and 10 mg/kg nano-selenium, respectively. The resuhs showed that, at 30 day, the blood selenium content was higher in the 2.5-10 mg/kg nano-selenium groups than that of other groups（P 〈 0.01）. The plasma T-AOC activities were higher in the 10 mg/ kg nano-selenium groups than that in other groups （P 〈 0.01）. groups than that in the control group （P 〈 0.01 or P 〈 0.05）. The plasma MDA content was lower in the nano-selenium The Plasma NO content in all nano-selenium groups was decreased （P 〈 0.01）. At 60 day, the blood selenium content was higher in the 2.5, 5.0 and 10 mg/kg nano-selenium group than in the control group and 0.3, 0.5 mg/kg nano-selenium groups（P 〈 0.01）. The plasma GSH-Px activities were lower in the 5.0 and 10 mg/kg nano-selenium groups than that in the other groups（P 〈 0.01）. The plasma NO content was higher in the 2.5-10 mg/kg nano-selenium groups than that of the other groups. To sum up, it is feasible that the ration in hen is supplemented with the nano-selenium 2.5 mg/kg.