牦牛mtDNA编码蛋白质的基因密码子偏好性研究及聚类分析

Codon Usage Bias Studies and Cluster Analysis on Protein Coding Genes of mtDNA in Bos grunniens

对牦牛线粒体DNA(mtDNA)编码蛋白质的基因序列进行分析,探讨基因序列密码子的使用模式和特性,并根据基因序列和密码子的偏好性对牦牛与其他物种间的进化关系进行聚类分析。结果表明,牦牛mtDNA编码蛋白质的基因偏爱使用碱基A以及以A结尾的密码子,UUC、UAC、CAC、CAA等28个密码子为牦牛mtDNA编码蛋白质基因的偏好性密码子。家牦牛与野牦牛亲缘关系最近,首先聚为一类,然后依次与其他牛、山羊、绵羊等物种相聚。

mtDNA protein coding gene sequences of Bos grunniens were analyzed with codon programe, and evolutionary relationship between Bos grunniens and other species was analyzed by a hierarchical cluster method in gene sequences and the relative synonymous codon usage frequency. The re suits showed that protein coding genes of mtDNA in Bos grunniens are tending to use the codons ending with A, and UUC,UAC,CAC,CAA et al. are preferable codons of mtDNA protein coding gene sequences in Bos grunniens, and there are similar preferences of codon usage between Bos grunniens mutus and Bos grunniens, but different from other species.