转DREB1A基因地被菊花Fall Color后代综合性状及耐寒性检测

Analysis of Comprehensive Traits and Cold Tolerance in DREB1A Transgenic Progeny of Chrysanthemum Fall Color

以转35S和rd29A启动子驱动的AtDREB1A基因地被菊花植株为试材,以组织培养微扦插的方式进行繁殖,对获得的转基因各株系无性后代进行性状观察及自然低温条件下的抗性检测。结果表明,转基因株系以茎段为外植体进行组织培养扩繁,植株在大田中移栽成活率高且能够正常生长,与野生型相比,转基因植株株高、冠幅、分枝角度性状存在表型差异,而花期、花色不存在表型差异;转基因各株系在低温胁迫下相对电导率和膜伤害率均明显降低,表明转基因株系增强了对低温的胁迫耐性;2007年冬季转基因株系存活率高,露地越冬能力也明显增强。

In the previous work,we have got the rd29A： AtDREB1A and 35S： AtDREBIA （Dehydration Responsive Element Binding Protein） transgenic groundcover chrysanthemum Fall Color, the lines have stronger tolerance to cold, drought and high salt stress than wild type （WT） plants. In this study,these transgenic lines propagated with tissue culture were used to observe the character and to test the tolerance under low temperature condition. The results showed that using the stem segments as explant,the propagated plants grew well with high transplant survival rate in field, compared with WT plants, the transgenie lines showed phenotypie differences in height, crown width, and branching angle but no differences in flowering time and flower color; the transgenic plants exhibited tolerance to cold stress with obviously lower relative electric conductivity and membrane damage rate of leaves as well as higher survival rate and enhanced overwintering ability in 2007.